PXD028969-1

PXD028969 is an original dataset announced via ProteomeXchange.

Title	Secretome profiling of Cryptosporidium sporozoites in the initial interaction processes with host epithelial cells
Description	Sporozoites secrete proteins which can help them adhere to/invade host cells during gliding motility on the surface of host cells. Currently, the composition of secreted proteins of C. parvum during adhesion/invasion of host cells is not fully understood. In this study, TMT labeled quantitative proteomics was used for the first time to compare and analyze the secreted proteins of C. parvum in the gliding motility state (3 hpi) and adhesion/invasion state (6 hpi). Bioinformatics analysis of the quantified differentially secreted proteins from C. parvum and HCT-8 cells showed that a total of 626 and 2339 C. parvum and HCT-8 cells secreted proteins were quantified. Differentially secreted proteins of C. parvum are mainly modified after translation, which are related to protein degradation and kinase activity, and cap-domain proteins are highly expressed during sporozoite gliding motility. However, differentially secreted proteins of HCT-8 cells are mainly concentrated in the pathways related to extracellular matrix and cell adhesion molecules. This study identified some important molecules that may be involved in the interaction between C. parvum and HCT-8 cells, providing basic data for the screening of key functional genes and the study of invasion mechanism of C. parvum.
HostingRepository	PRIDE
AnnounceDate	2025-11-17
AnnouncementXML	Submission_2025-11-16_16:19:37.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhaohui Cui
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Limnobaculum parvum; NCBI TaxID: NEWT:2172103;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-06 06:05:48	ID requested
⏵ 1	2025-11-16 16:19:38	announced

10.1021/acs.jproteome.5c00692;

Li N, Cui Z, Wang L, Sun S, Zhao W, Wang Y, Meng Q, Li X, Zhang S, Wu Y, Zhang L, Invasion. J Proteome Res, 24(11):5830-5841(2025) [pubmed]

submitter keyword: secrete proteins,Cryptospordium,Sporozoites

Zhaohui Cui
contact affiliation	College of Veterinary Medicine, Henan Agricultural University, International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou, China
contact email	czh9008@xcu.edu.cn
lab head
Zhaohui Cui
contact affiliation	College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
contact email	czh9008@xcu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD028969

PRIDE project URI

• PRIDE
  1. PXD028969
     1. Label: PRIDE project
     2. Name: Secretome profiling of Cryptosporidium sporozoites in the initial interaction processes with host epithelial cells