PXD028963-1

PXD028963 is an original dataset announced via ProteomeXchange.

Title	Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain
Description	In order to combat molecular damage, most cellular proteins undergo rapid turnover. We have previously identified large nuclear protein assemblies that can persist for years in post-mitotic tissues and are subject to age-related decline. Here we report that mitochondria can be long-lived in the mouse brain and reveal that specific mitochondrial proteins have half-lives longer than the average proteome. These mitochondrial long-lived proteins (mitoLLPs) are core components of the electron transport chain (ETC) and display increased longevity in respiratory supercomplexes. We find that COX7C, a mitoLLP that forms a stable contact site between Complexes I and IV, is required for Complex IV and supercomplex assembly. Remarkably, even upon depletion of COX7C transcripts, ETC function is maintained for days, effectively uncoupling mitochondrial function from ongoing transcription of its mitoLLPs. Our results suggest that modulating protein longevity within the ETC is critical for mitochondrial proteome maintenance and the robustness of mitochondrial function.
HostingRepository	PRIDE
AnnounceDate	2021-11-04
AnnouncementXML	Submission_2021-11-04_05:07:05.960.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028963
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Shefali Krishna
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-06 06:05:08	ID requested
⏵ 1	2021-11-04 05:07:06	announced

Dataset with its publication pending

submitter keyword: Mitochondria

protein homeostasis

long-lived proteins

electron transport chain

Martin Hetzer
contact affiliation	Salk Institute, La Jolla, CA USA
contact email	hetzer@salk.edu
lab head
Shefali Krishna
contact affiliation	Salk Institute of Biological Studies
contact email	skrishna@salk.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028963
     1. Label: PRIDE project
     2. Name: Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain