PXD028961-2

PXD028961 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative crotonylproteomics of mouse ovaries-Chronic resistant stress (CRS) and control group, two repeats each
Description	We found that in Chronic Resistant Stress (CRS)-treated mice, the fertility and oocyte quality significantly decreased, and plenty of biochemical indexes significantly changed, so we guessed that the overall physical health of CRS mice was severely impacted. As one major strategy to investigate the mechanism how CRS impact the physical health, We chose label-free quantitative ovarian crotonylproteomics service from Hangzhou PTM Bio Co. We sent two repeats of two-month-old CRS CD1/ICR mouse ovaries and two repeats of control ovaries for characterization of differentially crotonylated proteins. Each repeat contained about 60 ovaries from 30 female mice, about 300 mg. Gross protein levels were also examined and used to normalize the protein crotonylation level.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:41:02.797.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dong Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	crotonylated L-lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-06 04:19:08	ID requested
1	2024-05-22 02:02:18	announced
⏵ 2	2024-10-22 06:41:10	announced	2024-10-22: Updated project metadata.

Chen LJ, Tu ZY, Wang Y, He YH, Wang X, Tao SZ, Xu YY, Li CR, Wang RL, Yang ZX, Sun J, Ma X, Zhang D, ATP5O Hypo-crotonylation Caused by HDAC2 Hyper-Phosphorylation Is a Primary Detrimental Factor for Downregulated Phospholipid Metabolism under Chronic Stress. Research (Wash D C), 2022():9834963(2022) [pubmed]

10.34133/2022/9834963;

submitter keyword: Label-free quantitative crotonylproteomics, mouse ovaries, Chronic resistant stress

Dong Zhang
contact affiliation	State Key Lab of Reproductive Medicine, Nanjing Medical University
contact email	dong.ray.zhang@njmu.edu.cn
lab head
Dong Zhang
contact affiliation	State Key Lab of Reproductive Medicine, Nanjing Medical University
contact email	dong.ray.zhang@njmu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD028961

PRIDE project URI

• PRIDE
  1. PXD028961
     1. Label: PRIDE project
     2. Name: Label-free quantitative crotonylproteomics of mouse ovaries-Chronic resistant stress (CRS) and control group, two repeats each