PXD028958-1

PXD028958 is an original dataset announced via ProteomeXchange.

Title	Unravelling the initial triggers of Botrytis cinerea infections. Botrytis cinerea surfactome
Description	Botrytis cinerea, the causal agent of the gray mold, is one of the most important phytopathogenic fungi due to its ability to affect more than 1400 cultivable vegetal species. Signal transduction cascades mediate the dialogue between the environment, the plant and the fungus, which is essential in the infection process of B. cinerea. In this context, surface proteins (surfactome) have an important role as the first receptors in the signaling cascades, connecting the fungus response to the environment changes. Moreover, the relevant role of surfactome in infection process of pathogenic microorganism has been described, but nothing has been reported in B. cinerea. Therefore, in order to unravel new proteins and to complete the whole view of signal transduction cascades during infection of B. cinerea, surfactome of this fungus has been analyzed under two plant-based elicitors previously validated by the research group: glucose (GLU) as a constitutive stage and deproteinized tomato cell wall (TCW) as a virulence inductor. To identify the B. cinerea surfactome, the trypsin shaving of intact living cells approach has been optimized. Subsequently, all the peptide mixture obtained in surfactome isolation was analyzed using LC-MS/MS to identify their components. Our results shows that none of the used protocols disturbs the hyphae integrity, obtaining the best when PBS buffer plus 30% sucrose was used. In conclusion, in this work it has been carried out for the first time the optimization of a surface protein extraction protocol in Botrytis cinerea giving rise to a new subproteome; the surfactome. Integration of surfactome data inside the previous proteomics data collected by the research group has improved the complete view of signal transduction cascades during infection of B. cinerea, helping to unravel key points in the regulation of this process and therefore to further develop new and better discriminatory fungicides from a molecular approach.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_23:53:20.683.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028958
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sergio Ciordia
SpeciesList	scientific name: Botrytis cinerea B05.10; NCBI TaxID: 332648;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-06 02:00:15	ID requested
⏵ 1	2022-02-16 23:53:21	announced

Escobar-Ni, ñ, o A, Carrasco-Reinado R, Morano IM, Cantoral JM, Fernandez-Acero FJ, Infection: First Description of Its Surfactome. J Fungi (Basel), 7(12):(2021) [pubmed]

submitter keyword: Botrytis cinerea, proteome, Shaving, surfactome, virulence factor and pathogenicity factor

Francisco Javier Fernández Acero
contact affiliation	Microbiology Laboratory, Institute of Viticulture and Agri-food Research (IVAGRO), University of Cádiz, Pol. Río San Pedro s/n, Puerto Real, Cádiz, Spain
contact email	franciscojavier.fernandez@gm.uca.es
lab head
Sergio Ciordia
contact affiliation	Spanish National Center for Biotechnology
contact email	sciordia@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028958
     1. Label: PRIDE project
     2. Name: Unravelling the initial triggers of Botrytis cinerea infections. Botrytis cinerea surfactome