PXD028938 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ionophore 4-Br-A23187 causes copper stress in B. subtilis |
Description | Ionophores are small molecules or peptides that transport metal ions across biological membranes. Their transport capabilities are typically characterized in vitro using vesicles and single ion species. It is difficult to infer from these data which effects ionophores have on living cells in a complex environment (e.g. culture medium), since net transport is influenced e.g. by ion composition, concentration gradients, pH gradient, and active transport. To gain insights into the antibacterial mechanism of the semi-synthetic polyether ionophore 4-Br-A23187, we investigated its effects on the gram-positive model organism Bacillus subtilis. Changes in intracellular ion concentrations were determined using ionomics and the physiological impact was assessed by proteomics. 4-Br-A23187 treatment resulted in an increase in intracellular copper levels, an effect that was dependent on the copper concentration of the medium. The antibiotic effect of 4-Br-A23187 is further aggravated by a decrease in intracellular manganese and magnesium. Effects of copper hyperaccumulation, mirrored by the proteomic response, included oxidative stress, disturbance of proteostasis, metal, and sulfur homeostasis. Especially solvent-exposed iron-sulfur clusters like that of aconitase of the citric acid cycle are readily destabilized by copper. Using calcein fluorescence quenching as a readout, we confirmed in vitro that 4-Br-A23187 acts as copper ionophore. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-08 |
AnnouncementXML | Submission_2022-06-08_05:19:23.477.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christoph Senges |
SpeciesList | scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308; |
ModificationList | monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-04 10:03:20 | ID requested | |
⏵ 1 | 2022-06-08 05:19:23 | announced | |
2 | 2023-11-14 08:53:27 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: 4-Br-A23187, antibiotic, Bacillus subtilis, copper, ionophore |
Contact List
Julia E. Bandow |
contact affiliation | Applied Microbiology, Faculty of Biology and Biotechnology, Ruhr University Bochum, 44780 Bochum, Germany |
contact email | julia.bandow@rub.de |
lab head | |
Christoph Senges |
contact affiliation | Ruhr University Bochum |
contact email | christoph.senges@rub.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028938
- Label: PRIDE project
- Name: Ionophore 4-Br-A23187 causes copper stress in B. subtilis