PXD028902-2

PXD028902 is an original dataset announced via ProteomeXchange.

Title	Temporal and site-specific ADP-ribosylation dynamics upon different genotoxic stresses
Description	The DNA damage response revolves around transmission of information via post-translational modifications, including reversible protein ADP-ribosylation. Here, we applied a mass spectrometry-based Af1521 enrichment technology for the identification and quantification of ADP-ribosylation sites as a function of various DNA damage stimuli and time. In total, we detected 1,681 ADP-ribosylation sites residing on 716 proteins in U2OS cells, and determined their temporal dynamics after exposure to the genotoxins H2O2 and MMS. Intriguingly, we observed a widespread but low-abundant serine ADP-ribosylation response at the earliest time point, with later time points centered on increased modification of the same sites. This suggests that early serine ADP-ribosylation events may serve as a platform for an integrated signal response. While treatment with H2O2 and MMS induced homogenous ADP-ribosylation responses, we observed temporal differences in the ADP-ribosylation site abundancies. Exposure to MMS-induced alkylating stress induced the strongest ADP-ribosylome response after 30 minutes prominently modifying proteins involved in RNA processing, whereas in response to H2O2-induced oxidative stress ADP-ribosylation peaked after 60 minutes, while mainly modifying proteins involved in DNA damage pathways. Collectively, the dynamic ADP-ribosylome presented here provides valuable insight into the temporal cellular regulation of ADP-ribosylation in response to DNA damage.
HostingRepository	PRIDE
AnnounceDate	2023-05-14
AnnouncementXML	Submission_2023-05-14_14:19:03.280.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SaraLarsen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-04 04:44:57	ID requested
1	2021-11-04 00:31:08	announced
⏵ 2	2023-05-14 14:19:04	announced	2023-05-14: Updated project metadata.

Buch-Larsen SC, Rebak AKLFS, Hendriks IA, Nielsen ML, Temporal and Site-Specific ADP-Ribosylation Dynamics upon Different Genotoxic Stresses. Cells, 10(11):(2021) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: proteomics, mass spectrometry, PARP, Post-translational modification, DNA damage, Af1521,ADP-ribosylation

Michael LundNielsen
contact affiliation	Proteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark
contact email	michael.lund.nielsen@cpr.ku.dk
lab head
SaraLarsen
contact affiliation	NNF Center for Protein Research
contact email	sara.larsen@cpr.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028902
     1. Label: PRIDE project
     2. Name: Temporal and site-specific ADP-ribosylation dynamics upon different genotoxic stresses