PXD028867 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture |
Description | Precise control of gene expression during differentiation relies on the interplay of chromatin and nuclear structure. Despite an established contribution of nuclear membrane proteins to developmental gene regulation, little is known regarding the role of inner nuclear proteins. Here we demonstrate that loss of the nuclear scaffolding protein Matrin-3 (Matr3) in erythroid cells leads to morphological and gene expression changes characteristic of accelerated maturation, as well as broad alterations in chromatin organization similar to those accompanying differentiation. Matr3 protein interacts with CTCF and the cohesin complex, and its loss perturbs their occupancy at a subset of sites. Destabilization of CTCF and cohesin binding correlates with altered transcription and accelerated differentiation. This association is conserved in embryonic stem cells. Our findings indicate Matr3 negatively affects cell fate transitions and demonstrate that a critical inner nuclear protein impacts occupancy of architectural factors, culminating in broad effects on chromatin organization and cell differentiation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-04-11 |
AnnouncementXML | Submission_2023-04-11_06:08:42.881.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD028867 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Hye JiCha |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-29 15:01:57 | ID requested | |
1 | 2021-11-11 08:00:55 | announced | |
⏵ 2 | 2023-04-11 06:08:44 | announced | 2023-04-11: Updated project metadata. |
Publication List
Cha HJ, Uyan Ö, Kai Y, Liu T, Zhu Q, Tothova Z, Botten GA, Xu J, Yuan GC, Dekker J, Orkin SH, Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture. Nat Commun, 12(1):6241(2021) [pubmed] |
10.6019/PXD028867; |
Keyword List
Contact List
Stuart HOrkin |
contact affiliation | 1Division of Hematology/Oncology, Boston Children’s Hospital and Department of Pediatric Oncology, Dana-Farber Cancer Institute (DFCI), Harvard Stem Cell Institute, Harvard Medical School, Howard Hughes Medical Institute, Boston, MA 02115, USA |
contact email | stuart_orkin@dfci.harvard.edu |
lab head | |
Hye JiCha |
contact affiliation | Boston Children's Hospital |
contact email | hyeji.cha@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD028867 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028867
- Label: PRIDE project
- Name: Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture