PXD028851 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A substrate-like thrombin inhibitor from the saliva of the flea Xenopsylla cheopis is resistant to cleavage by the enzyme |
Description | XC-43 is a small peptide identified in the sialome of the flea Xenopsylla cheopis and act as a fast, tight-binding inhibitor of thrombin with a dissociation constant of less than 10 pM. The crystal structure of XC-43 in complex with thrombin shows that despite its substrate-like binding mode, XC-43 is not detectably cleaved by thrombin and that it interacts with the thrombin surface from the enzyme catalytic site through the fibrinogen-binding exosite I. The low rate of hydrolysis is verified in solution experiments with XC-43 which show the substrate to be largely intact after two hours of incubation with thrombin at 37°C. The potential of XC-43 as an anticoagulant is suggested by increased arterial occlusion time, tail bleeding time, and blood coagulation parameters in rat models of thrombosis. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-16 |
AnnouncementXML | Submission_2022-02-16_15:50:20.919.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stephen Lu |
SpeciesList | scientific name: Xenopsylla cheopis; NCBI TaxID: 163159; |
ModificationList | reduction of disulfide crosslink in cystine to two cysteines; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-29 13:59:19 | ID requested | |
⏵ 1 | 2022-02-16 15:50:21 | announced | |
Publication List
Lu S, Tirloni L, Oliveira MB, Bosio CF, Nardone GA, Zhang Y, Hinnebusch BJ, Ribeiro JM, Andersen JF, Identification of a substrate-like cleavage-resistant thrombin inhibitor from the saliva of the flea Xenopsylla cheopis. J Biol Chem, 297(5):101322(2021) [pubmed] |
Keyword List
submitter keyword: Blood feeding, X-ray crystallography, flea, thrombin |
Contact List
Jose M.C. Ribeiro |
contact affiliation | Laboratory of Malaria and Vector Research, NIAID, NIH |
contact email | JRIBEIRO@niaid.nih.gov |
lab head | |
Stephen Lu |
contact affiliation | NIH/NIAID |
contact email | stephen.lu@nih.gov |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD028851 |
PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD028851
- Label: PRIDE project
- Name: A substrate-like thrombin inhibitor from the saliva of the flea Xenopsylla cheopis is resistant to cleavage by the enzyme