PXD028818-1

PXD028818 is an original dataset announced via ProteomeXchange.

Title	How the presence of hemin affects the expression of all iron-uptake pathways in Pseudomonas aeruginosa cells
Description	Iron is an essential nutriment for almost all organisms, but this metal is poorly bioavailable. During infection, bacteria access iron from the host by importing either iron or hemin. Pseudomonas aeruginosa, a Gram-negative pathogen, secretes two siderophores, pyoverdine (PVD) and pyochelin (PCH), to access iron and is also able to use many siderophores produced by other microorganisms (called xenosiderophores). To access hemin, P. aeruginosa uses three distinct uptake pathways, named Has, Phu, and Hxu. We previously showed that P. aeruginosa expresses the Has and Phu hemin uptake systems, as well as the PVD- and PCH-dependent iron-uptake pathways, in iron-restricted growth conditions using proteomic and RT-qPCR approaches. Here, using the same approach, we show that physiological concentrations of hemin in the bacterial growth medium result in the repression of the expression of the proteins of the PVD- and PCH-dependent iron-uptake pathways, leading to less production of these two siderophores. This indicates that the pathogen adapts its phenotype to use hemin as an iron source rather than produce PVD and PCH to access iron. Moreover, the presence of both hemin and a xenosiderophore resulted in (i) the strong induction of the expression of the proteins of the added xenosiderophore-uptake pathway, (ii) repression of the PVD- and PCH-dependent iron-uptake pathways, and (iii) no effect on the expression levels of the Has, Phu, or Hxu systems, indicating that bacteria use both xenosiderophores and hemin to access iron.
HostingRepository	PRIDE
AnnounceDate	2022-03-15
AnnouncementXML	Submission_2022-03-15_01:41:20.104.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028818
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lauriane Kuhn
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-28 10:08:27	ID requested
⏵ 1	2022-03-15 01:41:20	announced

Normant V, Kuhn L, Munier M, Hammann P, Mislin GLA, Schalk IJ, Cells. ACS Infect Dis, 8(1):183-196(2022) [pubmed]

submitter keyword: Iron uptake pathway, Pseudomonas, hemin

Isabelle Schalk
contact affiliation	CNRS, UMR7242, UMR7242, ESBS, Bld Sébastien Brant, F-67412 Illkirch, Strasbourg, France
contact email	isabelle.schalk@unistra.fr
lab head
Lauriane Kuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028818
     1. Label: PRIDE project
     2. Name: How the presence of hemin affects the expression of all iron-uptake pathways in Pseudomonas aeruginosa cells