PXD028808-1

PXD028808 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis thaliana trichome proteome
Description	Trichomes of Arabidopsis thaliana have been broadly used to study cell development, cell differentiation and cell wall biogenesis. In this context, the exposed position, extraordinary size and characteristic morphology of trichomes featured particularly the exploration of trichome mutant phenotypes. However, trichome-specific biochemical or -omics analyses require a proper separation of trichomes from residual plant tissue. Thus, different strategies were proposed in the past for trichome isolation, which mostly rely on harsh conditions. To improve trichome-leaf separation, we revised a previously proposed method for isolating A. thaliana trichomes by optimizing the mechanical and biochemical specifications for trichome release. Furthermore, we introduced a density gradient centrifugation step to remove residual plant debris. We found that prolonged, yet mild seedling agitation increases the overall trichome yield by about 62% compared to the original protocol. We noticed that subsequent density gradient centrifugation further visually enhances trichome purity, which could be advantageous for downstream analyses. Histochemical and biochemical investigation of trichome cell wall composition indicated that gentle agitation during trichome release largely preserves trichome integrity. We used enriched and purified trichomes for proteomic analysis and present a reference data set of trichome-resident and -enriched proteins.
HostingRepository	PRIDE
AnnounceDate	2022-01-13
AnnouncementXML	Submission_2022-01-13_03:17:01.297.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pitter Huesgen
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-28 09:40:22	ID requested
⏵ 1	2022-01-13 03:17:01	announced

Dataset with its publication pending

submitter keyword: plant, trichome, arabiodpsis thaliana, proteome

Pitter Huesgen
contact affiliation	ZEA-3 Forschungszentrum Jülich Germany
contact email	p.huesgen@fz-juelich.de
lab head
Pitter Huesgen
contact affiliation	Forschungszentrum Jülich
contact email	p.huesgen@fz-juelich.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028808
     1. Label: PRIDE project
     2. Name: Arabidopsis thaliana trichome proteome