PXD028793 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Peptidomic changes in the milk of water buffaloes (Bubalus bubalis) with intramammary infection by non-aureus staphylococci |
Description | Infectious mastitis by non-aureus staphylococci (NAS) is a significant issue in dairy buffalo farming. In a herd with subclinical NAS mastitis, we identified Staphylococcus microti as the predominant species by MALDI-TOF mass spectrometry. To assess milk protein integrity and investigate potential disease markers, we characterized 12 NAS-positive and 12 healthy quarter milk samples by shotgun peptidomics combining peptide enrichment and high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS). We observed significant changes in the milk peptidome. Out of 789 total peptides identified in each group, 49 and 44 were unique or increased in NAS-positive and healthy milk, respectively. In terms of sequence, these belonged mainly to caseins in NAS-positive milk, followed by milk fat globule membrane proteins (MFGMP) and by the immune defense/antimicrobial proteins osteopontin, lactoperoxidase, and serum amyloid A. In healthy milk, the differential peptides belonged mainly to MFGMP, followed by caseins. In terms of abundance, peptides from MFGMP and immune defense protein were higher in NAS-positive milk, while peptides from caseins were higher in healthy milk. These findings highlight the impact of NAS on buffalo milk quality and mammary gland health, even when clinical signs are not evident, and underscore the need for clarifying the epidemiology and relevance of the different NAS species in this dairy ruminant. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-09 |
AnnouncementXML | Submission_2022-06-09_04:19:35.739.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Francesca Grassi Scalvini |
SpeciesList | scientific name: Bubalus bubalis; NCBI TaxID: 89462; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-27 07:23:11 | ID requested | |
⏵ 1 | 2022-06-09 04:19:36 | announced | |
Publication List
Addis MF, Maffioli EM, Penati M, Albertini M, Bronzo V, Piccinini R, Tangorra F, Tedeschi G, Cappelli G, Di Vuolo G, Vecchio D, De Carlo E, Ceciliani F, Peptidomic changes in the milk of water buffaloes (Bubalus bubalis) with intramammary infection by non-aureus staphylococci. Sci Rep, 12(1):8371(2022) [pubmed] |
Keyword List
submitter keyword: nano-LC-MS/MS |
Contact List
Francesca Grassi Scalvini |
contact affiliation | department of veterinary medicine |
contact email | francesca.grassiscalvini@guest.unimi.it |
lab head | |
Francesca Grassi Scalvini |
contact affiliation | Università degli studi di Milano |
contact email | francesca.grassiscalvini@guest.unimi.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028793
- Label: PRIDE project
- Name: Peptidomic changes in the milk of water buffaloes (Bubalus bubalis) with intramammary infection by non-aureus staphylococci