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PXD028793-1

PXD028793 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePeptidomic changes in the milk of water buffaloes (Bubalus bubalis) with intramammary infection by non-aureus staphylococci
DescriptionInfectious mastitis by non-aureus staphylococci (NAS) is a significant issue in dairy buffalo farming. In a herd with subclinical NAS mastitis, we identified Staphylococcus microti as the predominant species by MALDI-TOF mass spectrometry. To assess milk protein integrity and investigate potential disease markers, we characterized 12 NAS-positive and 12 healthy quarter milk samples by shotgun peptidomics combining peptide enrichment and high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS). We observed significant changes in the milk peptidome. Out of 789 total peptides identified in each group, 49 and 44 were unique or increased in NAS-positive and healthy milk, respectively. In terms of sequence, these belonged mainly to caseins in NAS-positive milk, followed by milk fat globule membrane proteins (MFGMP) and by the immune defense/antimicrobial proteins osteopontin, lactoperoxidase, and serum amyloid A. In healthy milk, the differential peptides belonged mainly to MFGMP, followed by caseins. In terms of abundance, peptides from MFGMP and immune defense protein were higher in NAS-positive milk, while peptides from caseins were higher in healthy milk. These findings highlight the impact of NAS on buffalo milk quality and mammary gland health, even when clinical signs are not evident, and underscore the need for clarifying the epidemiology and relevance of the different NAS species in this dairy ruminant.
HostingRepositoryPRIDE
AnnounceDate2022-06-09
AnnouncementXMLSubmission_2022-06-09_04:19:35.739.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrancesca Grassi Scalvini
SpeciesList scientific name: Bubalus bubalis; NCBI TaxID: 89462;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-27 07:23:11ID requested
12022-06-09 04:19:36announced
Publication List
Addis MF, Maffioli EM, Penati M, Albertini M, Bronzo V, Piccinini R, Tangorra F, Tedeschi G, Cappelli G, Di Vuolo G, Vecchio D, De Carlo E, Ceciliani F, Peptidomic changes in the milk of water buffaloes (Bubalus bubalis) with intramammary infection by non-aureus staphylococci. Sci Rep, 12(1):8371(2022) [pubmed]
Keyword List
submitter keyword: nano-LC-MS/MS
Contact List
Francesca Grassi Scalvini
contact affiliationdepartment of veterinary medicine
contact emailfrancesca.grassiscalvini@guest.unimi.it
lab head
Francesca Grassi Scalvini
contact affiliationUniversità degli studi di Milano
contact emailfrancesca.grassiscalvini@guest.unimi.it
dataset submitter
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