PXD028793-1

PXD028793 is an original dataset announced via ProteomeXchange.

Title	Peptidomic changes in the milk of water buffaloes (Bubalus bubalis) with intramammary infection by non-aureus staphylococci
Description	Infectious mastitis by non-aureus staphylococci (NAS) is a significant issue in dairy buffalo farming. In a herd with subclinical NAS mastitis, we identified Staphylococcus microti as the predominant species by MALDI-TOF mass spectrometry. To assess milk protein integrity and investigate potential disease markers, we characterized 12 NAS-positive and 12 healthy quarter milk samples by shotgun peptidomics combining peptide enrichment and high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS). We observed significant changes in the milk peptidome. Out of 789 total peptides identified in each group, 49 and 44 were unique or increased in NAS-positive and healthy milk, respectively. In terms of sequence, these belonged mainly to caseins in NAS-positive milk, followed by milk fat globule membrane proteins (MFGMP) and by the immune defense/antimicrobial proteins osteopontin, lactoperoxidase, and serum amyloid A. In healthy milk, the differential peptides belonged mainly to MFGMP, followed by caseins. In terms of abundance, peptides from MFGMP and immune defense protein were higher in NAS-positive milk, while peptides from caseins were higher in healthy milk. These findings highlight the impact of NAS on buffalo milk quality and mammary gland health, even when clinical signs are not evident, and underscore the need for clarifying the epidemiology and relevance of the different NAS species in this dairy ruminant.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_04:19:35.739.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesca Grassi Scalvini
SpeciesList	scientific name: Bubalus bubalis; NCBI TaxID: 89462;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-27 07:23:11	ID requested
⏵ 1	2022-06-09 04:19:36	announced

Addis MF, Maffioli EM, Penati M, Albertini M, Bronzo V, Piccinini R, Tangorra F, Tedeschi G, Cappelli G, Di Vuolo G, Vecchio D, De Carlo E, Ceciliani F, Peptidomic changes in the milk of water buffaloes (Bubalus bubalis) with intramammary infection by non-aureus staphylococci. Sci Rep, 12(1):8371(2022) [pubmed]

submitter keyword: nano-LC-MS/MS

Francesca Grassi Scalvini
contact affiliation	department of veterinary medicine
contact email	francesca.grassiscalvini@guest.unimi.it
lab head
Francesca Grassi Scalvini
contact affiliation	Università degli studi di Milano
contact email	francesca.grassiscalvini@guest.unimi.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028793
     1. Label: PRIDE project
     2. Name: Peptidomic changes in the milk of water buffaloes (Bubalus bubalis) with intramammary infection by non-aureus staphylococci