PXD028688-1

PXD028688 is an original dataset announced via ProteomeXchange.

Title	Integrative analysis of transcriptome and proteome provides insights into adaptation to cadmium stress in Sedum plumbizincicola
Description	Sedum plumbizincicola, a cadmium (Cd) hyperaccumulating herbaceous plant, can accumulate large amounts of Cd in the above-ground tissues without being poisoned. However, the molecular mechanisms regulating the processes are not fully understood. In this study, Transcriptional and proteomic analyses were integrated to investigate the response of S. plumbizincicola plants to cadmium stress and to identify key pathways that are potentially responsible for Cd tolerance and accumulation. A total of 6064 proteins were identified from Tandem Mass Tag (TMT) based proteome, among which 630 DAPs (differentially abundant proteins) using fold change >1.5 with p-value <0.05 were involved in processes including phenylpropanoid biosynthesis, protein processing in endoplasmic reticulum, and biosynthesis of secondary metabolites. Combined with the previous transcriptomic study, 209 corresponding genes and proteins showed the identical expression pattern. The identified genes/proteins revealed the regulatory roles of several metabolism pathways, such as phenylpropanoid biosynthesis, oxidative phosphorylation, phagosome, and glutathione metabolism, in mediating Cd tolerance and accumulation. Functional characterization of a Cd up regulated gene SpFAOMT provided insight into its role in lignin biosynthesis in S.plumbizincicola during Cd stress.
HostingRepository	iProX
AnnounceDate	2021-09-21
AnnouncementXML	Submission_2022-01-25_05:17:25.538.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhiping Deng
SpeciesList	scientific name: Sedum plumbizincicola; NCBI TaxID: 1532924;
ModificationList	TMT6plex reporter fragment
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-21 19:36:50	ID requested
⏵ 1	2022-01-25 05:17:26	announced

Zhu Y, Qiu W, He X, Wu L, Bi D, Deng Z, He Z, Wu C, Zhuo R, analysis of transcriptome and proteome provides insights into adaptation to cadmium stress in Sedum plumbizincicola. Ecotoxicol Environ Saf, 230():113149(2022) [pubmed]

submitter keyword: Sedum plumbizincicola, cadmium, transcriptome, proteome

Renying Zhuo
contact affiliation	Research Institute of Subtropical Forestry, Chinese Academy of Forestry
contact email	zhuory@gmail.com
lab head
zhiping Deng
contact affiliation	Zhejiang Academy of Agricultural Sciences
contact email	zhipingdeng@126.com
dataset submitter

iProX dataset URI