PXD028661-1

PXD028661 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The NuRD complex is required for Thpok-mediated CD4+ T cell development
Description	This data represent characterization of the interactome of murine Thpok. A version of Thpok (Thpok-Bio) subject to biotinylation in vivo by the BirA biotin ligase was used; to assess Thpok interactions in primary cells, Thpok-Bio was retrovirally expressed in in vitro activated CD4+ T cells from Thpokfl/fl Ox40-Cre+ mice carrying a Rosa26BirA allele. Expression of Ox40-Cre, initiated upon T cell activation, causes the deletion of endogenous Thpokfl alleles, so that transduced cells only express Thpok-Bio. Two Thpok deletion constructs (BKK and RFK) were also analyzed. Following streptavidin pulldown, proteins were separated by SDS-PAGE, the full lane cut into 9 slices, and in-gel digested. FIles are: 1384 - empty vector; 1385 - Thpok; 1386 - Thpok + Ethidium Bromide; 1387 - Thpok BKK; 1388 - Thpok RFK.
HostingRepository	MassIVE
AnnounceDate	2022-11-15
AnnouncementXML	Submission_2022-11-15_07:07:25.283.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lisa Jenkins
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-20 10:20:59	ID requested
⏵ 1	2022-11-15 07:07:25	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Thpok, Interactome

submitter keyword: Thpok, Interactome

Contact List

Lisa Jenkins
contact affiliation	NCI, NIH
contact email	jenkinsl@mail.nih.gov
lab head
Lisa Jenkins
contact affiliation	NCI
contact email	jenkinsl@mail.nih.gov
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088126/