PXD028629-1
PXD028629 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Northern elephant seal skeletal muscle, blubber, and plasma proteome responses to prolonged fasting |
| Description | Northern elephant seal skeletal muscle, blubber, and plasma were collected from 5 adult females early and late in a 5-week molting fast (mean 33 days apart; see Sample Key in supplementary files). Proteins were isolated from muscle using the Trizol method and blubber using Qiazol with the Qiagen RNeasy Lipid Mini kit (see Khudyakov et al., 2018, doi: 10.1242/bio.036731). Plasma samples were used raw (without depletion of highly abundant proteins). Muscle, blubber, and plasma proteins were denatured using 1% sodium deoxycholate, 8 M urea, and 5 mM DTT in 50 mM ammonium bicarbonate and digested in-solution using trypsin (1:50 trypsin to protein ratio). |
| HostingRepository | MassIVE |
| AnnounceDate | 2021-09-19 |
| AnnouncementXML | Submission_2021-09-19_15:16:34.239.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jane Khudyakov |
| SpeciesList | scientific name: Mirounga angustirostris; common name: Northern elephant seal; NCBI TaxID: 9716; |
| ModificationList | Carbamidomethyl; Oxidation; Deamidated |
| Instrument | Orbitrap Fusion ETD |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-09-19 14:28:07 | ID requested | |
| ⏵ 1 | 2021-09-19 15:16:34 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: fasting, proteome, seal, muscle, blubber, plasma |
Contact List
| Jane Khudyakov | |
|---|---|
| contact affiliation | University of the Pacific |
| contact email | jkhudyakov@pacific.edu |
| lab head | |
| Jane Khudyakov | |
| contact affiliation | University of the Pacific |
| contact email | jkhudyakov@pacific.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088122/ |
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