PXD028613-1

PXD028613 is an original dataset announced via ProteomeXchange.

Title	Muc2-dependent microbial colonization of the jejunal mucus layer is diet sensitive and confers resistance to enteric pathogen infection
Description	Obesity is associated with an increased risk of mucosal infections; however, the mechanistic basis of this phenomenon remains incompletely defined. Intestinal mucus barrier systems normally prevent infections, but are sensitive to changes in the luminal environment. Here we demonstrate that mice exposed to an obesogenic Western-style diet (WSD) suffer regiospecific failure of the mucus barrier in the small intestinal jejunum caused by diet-induced mucus condensation, which occurs independently of microbiota alterations. Mucus barrier disruption due to either WSD exposure or chromosomal Muc2 deletion results in collapse of the commensal jejunal microbiota, which in turn sensitises mice to atypical jejunal colonization by the enteric pathogen Citrobacter rodentium. We identify the jejunal mucus layer as a microbial habitat, and link the regiospecific mucus dependency of the microbiota to fundamental properties of the jejunal niche. Together, our data identifies a symbiotic mucus-microbiota relationship that normally prevents jejunal pathogen colonization, but is highly sensitive to disruption by exposure to a Western-style diet.
HostingRepository	PRIDE
AnnounceDate	2023-05-12
AnnouncementXML	Submission_2023-05-12_07:20:43.090.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	LiisaArike
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-17 09:10:01	ID requested
⏵ 1	2023-05-12 07:20:43	announced
2	2023-11-14 08:53:28	announced	2023-11-14: Updated project metadata.

submitter keyword: proteomics, Q-Exactive, obesity,Mucus, mouse

LiisaArike
contact affiliation	Proteomics laboratory, Mucin Biology Groups, Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Sweden
contact email	liisaarike@gmail.com
lab head
LiisaArike
contact affiliation	PhD
contact email	liisaarike@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028613
     1. Label: PRIDE project
     2. Name: Muc2-dependent microbial colonization of the jejunal mucus layer is diet sensitive and confers resistance to enteric pathogen infection