PXD028594-1

PXD028594 is an original dataset announced via ProteomeXchange.

Title	Radiotherapy changes the salivary proteome in head and neck cancer patients: evaluation before, during and after treatment
Description	Objectives: Salivary glands are affected during radiotherapy in the head and neck region, leading to a reduction in salivary flow and changes its composition. Besides negatively affecting the oral soft tissues, this can also lead to dental impairment. Thus, we evaluated the effect of radiotherapy in the proteomic profile of the saliva in patients with head-and-neck-cancer (HNC). Materials and methods: HNC patients had their saliva collected before (BRT), during (2-5 weeks; DRT) and after (3-4 months; ART) radiotherapy. Saliva was also collected from healthy volunteers (control; C). Samples were processed for proteomic analysis. Results: In total 1.055 proteins were identified, among which 46 were common to all groups, while 86, 86, 286 and 395 were exclusively found in C, BRT, DRT and ART, respectively. Remarkably, alpha-enolase was increased 35-fold DRT compared with BRT, while proline-rich proteins were decreased. ART there was a 16-fold increase in scaffold attachment factor-B1 and a 3-fold decrease in alpha-enolase and several cystatins. When compared with C, salivary proteins of BRT patients showed increases cystatin-C, lysozyme C, histatin-1 and proline-rich proteins. Conclusion/Clinical revelance: Both HNC and radiotherapy remarkably change the salivary protein composition. Altogether, our results, for the first time, suggest investigating alpha-enolase levels in saliva DRT in future studies as a possible biomarker and strategy to predict the efficiency of the treatment. Moreover, our data provide important insights for designing dental products that are more effective for these patients and contribute to a better understanding of the progressive changes in salivary proteins induced by radiotherapy.
HostingRepository	PRIDE
AnnounceDate	2021-10-12
AnnouncementXML	Submission_2021-10-12_05:51:33.638.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Talita Ventura
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Xevo G2 Q-Tof; nanoACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 22:56:55	ID requested
⏵ 1	2021-10-12 05:51:33	announced

Dataset with its publication pending

submitter keyword: saliva, proteomics, head and neck cancer, radiotherapy, biomarkes, dental caries

Talita Mendes Oliveira Ventura
contact affiliation	Department of Biological Science - Laboratory of Biochemistry. Bauru School of Dentistry - University of São Paulo.
contact email	talitaventura@usp.br
lab head
Talita Ventura
contact affiliation	University of São Paulo, Bauru Dental School
contact email	talitaventura@usp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028594
     1. Label: PRIDE project
     2. Name: Radiotherapy changes the salivary proteome in head and neck cancer patients: evaluation before, during and after treatment