PXD028576-1

PXD028576 is an original dataset announced via ProteomeXchange.

Title	The mature endosperm governs early seedling development: a focus on cuticle formation
Description	In Arabidopsis mature seeds, the onset of the embryo-to-seedling transition is nonautonomously controlled, being blocked by endospermic abscisic acid (ABA) release under unfavorable conditions. Mature embryos lack an impermeable cuticle, unlike seedlings, consistent with their endospermic ABA uptake capability. Seedling cuticle formation occurs after germination rather than during embryogenesis. Mature endosperm removal prevents seedling cuticle formation and seed reconstitution by endosperm grafting onto embryos shows that the endosperm promotes seedling cuticle development. Grafting different endosperm and embryo mutant combinations, together with biochemical, microscopy and mass spectrometry approaches, reveals that endospermic release of Tyrosyl Sulfate Transferase (TPST)-sulfated CIF2 and PSY1 peptides promotes seedling cuticle development. Endosperm-deprived embryos produced nonviable seedlings bearing numerous developmental defects, in a manner unrelated to embryo nourishment, all restored by exogenously provided endosperm. Hence, seedling establishment is nonautonomous, requiring the mature endosperm.
HostingRepository	PRIDE
AnnounceDate	2021-11-10
AnnouncementXML	Submission_2021-11-10_07:49:06.128.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028576
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Patrice Waridel
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	sulfated residue; pentosylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 08:59:32	ID requested
⏵ 1	2021-11-10 07:49:06	announced
2	2022-01-04 15:54:14	announced	2022-01-04: Updated project metadata.

Dataset with its publication pending

submitter keyword: Arabidopsis, seed, endosperm, cuticle, development, PSY1

Luis Lopez-Molina
contact affiliation	Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland.
contact email	Luis.LopezMolina@unige.ch
lab head
Patrice Waridel
contact affiliation	University of Lausanne
contact email	patrice.waridel@unil.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028576
     1. Label: PRIDE project
     2. Name: The mature endosperm governs early seedling development: a focus on cuticle formation