PXD028563-1

PXD028563 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of different pgr5 mutants and pgrl1ab in Arabidopsis thaliana
Description	PGR5 is a small chloroplast protein that performs cyclic electron flow (CEF) around photosystem I and it is required for the proton gradient generation across the thylakoid membrane. The level of this protein is closely regulated by PGRL1. However, the molecular mechanism of PGR5 is still elusive. In this work, we have generated a new pgr5 mutant in Arabidopsis using CRISPR-Cas9 technology, pgr5-Cas, completely devoid of PGR5. We performed comparative proteomic analysis on pgr5-1 and pgr5-Cas lines (#1 and #2), as well as on pgrl1ab mutant.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_03:20:30.652.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giada Marino
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 07:40:36	ID requested
⏵ 1	2022-08-12 03:20:31	announced

Penzler JF, Marino G, Reiter B, Kleine T, Naranjo B, Leister D, Commonalities and specialties in photosynthetic functions of PROTON GRADIENT REGULATION5 variants in Arabidopsis. Plant Physiol, 190(3):1866-1882(2022) [pubmed]

submitter keyword: Arabidopsis, chloroplast, IMPACTII, Bruker, LFQ

Dario Leister
contact affiliation	Ludwig-Maximilians-University Munich (LMU), Faculty of Biology, Chair of Plant Molecular Biology (Botany), Großhaderner Str. 2, D-82152 Planegg-Martinsried,Germany
contact email	leister@lmu.de
lab head
Giada Marino
contact affiliation	Ludwig-Maximilians-University Munich (LMU) Department Biology I Plant Molecular Biology (Botany)
contact email	giada.marino@lmu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028563
     1. Label: PRIDE project
     2. Name: Proteomic analysis of different pgr5 mutants and pgrl1ab in Arabidopsis thaliana