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PXD028563-1

PXD028563 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of different pgr5 mutants and pgrl1ab in Arabidopsis thaliana
DescriptionPGR5 is a small chloroplast protein that performs cyclic electron flow (CEF) around photosystem I and it is required for the proton gradient generation across the thylakoid membrane. The level of this protein is closely regulated by PGRL1. However, the molecular mechanism of PGR5 is still elusive. In this work, we have generated a new pgr5 mutant in Arabidopsis using CRISPR-Cas9 technology, pgr5-Cas, completely devoid of PGR5. We performed comparative proteomic analysis on pgr5-1 and pgr5-Cas lines (#1 and #2), as well as on pgrl1ab mutant.
HostingRepositoryPRIDE
AnnounceDate2022-08-12
AnnouncementXMLSubmission_2022-08-12_03:20:30.652.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGiada Marino
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrumentimpact II
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-16 07:40:36ID requested
12022-08-12 03:20:31announced
Publication List
Penzler JF, Marino G, Reiter B, Kleine T, Naranjo B, Leister D, Commonalities and specialties in photosynthetic functions of PROTON GRADIENT REGULATION5 variants in Arabidopsis. Plant Physiol, 190(3):1866-1882(2022) [pubmed]
Keyword List
submitter keyword: Arabidopsis, chloroplast, IMPACTII, Bruker, LFQ
Contact List
Dario Leister
contact affiliationLudwig-Maximilians-University Munich (LMU), Faculty of Biology, Chair of Plant Molecular Biology (Botany), Großhaderner Str. 2, D-82152 Planegg-Martinsried,Germany
contact emailleister@lmu.de
lab head
Giada Marino
contact affiliationLudwig-Maximilians-University Munich (LMU) Department Biology I Plant Molecular Biology (Botany)
contact emailgiada.marino@lmu.de
dataset submitter
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Dataset FTP location
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