PXD028551-2
PXD028551 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Capacitive electrical stimulation effects on osteoblast-released matrix microvesicles |
| Description | Passive implants currently used in the clinics cannot prevent failure rates and inherent revision arthroplasties. Novel bioelectronic devices that include biophysical stimulators (such as electric stimulators) and sensing systems are desired since these will allow for long-term monitoring and control of the bone-implant interface, in a personalized manner. We have developed acting-sensing dual systems operated at high frequency (HF) that are able to stimulate osteoconduction in pre-osteoblasts and osteoinduction in human adipose-derived mesenchymal stem cells (hASCs). The project included – to our knowledge – the first-time proteomic analysis of microvesicles secreted from osteoblasts electrically-stimulated in vitro by a capacitive stimulator of thin interdigitated electrodes delivering an electrical 60 kHz HF stimulation, 30 min/day. Results revealed regulation of osteodifferentiation and mineralization-related proteins (e.g. Tgfb3, Ttyh3, Itih1, Aldh1a1). |
| HostingRepository | PRIDE |
| AnnounceDate | 2021-11-29 |
| AnnouncementXML | Submission_2021-12-03_01:30:44.710.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD028551 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Bárbara M de Sousa |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-09-16 07:39:07 | ID requested | |
| 1 | 2021-11-29 06:20:07 | announced | |
| ⏵ 2 | 2021-12-03 01:30:45 | announced | 2021-12-03: Updated publication reference for DOI(s): 10.1038/s41536-021-00184-6. |
| 3 | 2021-12-15 07:30:25 | announced | 2021-12-03: Updated publication reference for DOI(s): 10.1038/s41536-021-00184-6. 2021-12-15: Updated publication reference for PubMed record(s): 34815414. 2021-12-15: Updated publication reference for DOI(s): 10.1038/s41536-021-00184-6. |
| 4 | 2023-11-14 08:52:41 | announced | 2023-11-14: Updated project metadata. |
Publication List
| 10.1038/s41536-021-00184-6; |
Keyword List
| submitter keyword: extracellular, matrix microvesicles, osteoblasts, capacitive coupling, electrical stimulation, bone, LC-MS/MS |
Contact List
| Sandra I. Vieira | |
|---|---|
| contact affiliation | Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal. |
| contact email | sivieira@ua.pt |
| lab head | |
| Bárbara M de Sousa | |
| contact affiliation | Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, Portugal |
| contact email | bmsousa@ua.pt |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD028551 |
| PRIDE project URI |
Repository Record List
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