PXD028527 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Paulinella chromatophore N-terminome |
Description | The cercozoan amoeba Paulinella chromatophora contains photosynthetic organelles - termed chromatophores - that evolved from a cyanobacterium ~100 million years ago, independently from plastids in plants and algae. Despite its more recent origin, at least one third of the chromatophore proteome consists of nucleus-encoded proteins that are imported by an unknown mechanism across the chromatophore double envelope membranes. Chromatophore-targeted proteins fall into two classes. Proteins exceeding 250 amino acids carry a conserved N-terminal sequence extension, termed the ‘chromatophore transit peptide’ (crTP), that is presumably involved in guiding these proteins into the chromatophore. Short imported proteins do not carry discernable targeting signals. To explore whether the import of protein is accompanied by their N-terminal processing, here we used a mass spectrometry-based approach to determine protein N-termini in Paulinella chromatophora and identified N-termini of 208 chromatophore-localized proteins. Our study revealed extensive N-terminal modifications by acetylation and proteolytic processing in both, the nucleus and chromatophore-encoded fraction of the chromatophore proteome. Mature N-termini of 37 crTP-carrying proteins were identified, of which 30 were cleaved in a common processing region. Our results imply that the crTP mediates trafficking through the Golgi, is bipartite and surprisingly only the N-terminal third (‘part 1’) becomes cleaved upon import, whereas the rest (‘part 2’) remains at the mature proteins. In contrast, short imported proteins remain largely unprocessed. Finally, this work sheds light on N-terminal processing of proteins encoded in an evolutionary-early-stage photosynthetic organelle and suggests host-derived post-translationally acting factors involved in dynamic regulation of the chromatophore-encoded chromatophore proteome. |
HostingRepository | PRIDE |
AnnounceDate | 2023-04-06 |
AnnouncementXML | Submission_2023-04-06_06:32:26.006.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | PitterHuesgen |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | maXis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-16 06:41:32 | ID requested | |
⏵ 1 | 2023-04-06 06:32:26 | announced | |
2 | 2023-11-14 07:45:38 | announced | 2023-11-14: Updated project metadata. |
Publication List
Oberleitner L, Perrar A, Macorano L, Huesgen PF, Nowack ECM, A bipartite chromatophore transit peptide and N-terminal protein processing in the Paulinella chromatophore. Plant Physiol, 189(1):152-164(2022) [pubmed] |
Keyword List
submitter keyword: protein import,chromatophore, Paulinella chromatophore |
Contact List
PitterHuesgen |
contact affiliation | ZEA-3 Analytics, Forschungszentrum Juelich |
contact email | p.huesgen@fz-juelich.de |
lab head | |
PitterHuesgen |
contact affiliation | Forschungszentrum Jülich |
contact email | p.huesgen@fz-juelich.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028527
- Label: PRIDE project
- Name: The Paulinella chromatophore N-terminome