PXD028509-1

PXD028509 is an original dataset announced via ProteomeXchange.

Title	H. pylori utilizes membrane vesicles as a novel tool to extrude bismuth drug
Description	Antibiotic resistance is one of the major threats to human health. Combination of antibiotics with metals is among the proposed alternative treatments. However, so far only one such combination with the metal bismuth successfully used in clinics against Helicobacter pylori. This bacterial pathogen colonizes the human stomach potentially resulting in gastric cancer that causes 800,000 deaths every year. The effect of bismuth on H. pylori is not understood, for sub-lethal doses, such as those present in the plasma of treated patients. We addressed this question and observed that bismuth induces the formation of homogeneous membrane vesicles (MVs) with unique protein cargo content enriched in bismuth-binding proteins. To our surprise, MVs concentrate bismuth, unlike bacterial cells from which they originate. Our results revealed a novel function of MVs in metal detoxification where bismuth is accumulated to discard it from bacteria. Polyphosphate granules (poly-P) formed upon bismuth exposure are associated with changes in nucleoid structure and cell segregation. Thus, even low doses of bismuth induce profound changes in H. pylori physiology and highlight novel defense mechanisms employed by bacteria against bismuth toxicity.
HostingRepository	PRIDE
AnnounceDate	2022-09-19
AnnouncementXML	Submission_2022-09-19_02:29:14.689.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magalie Duchateau
SpeciesList	scientific name: Helicobacter pylori G27; NCBI TaxID: 563041;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 02:32:05	ID requested
⏵ 1	2022-09-19 02:29:15	announced
2	2023-11-14 08:52:40	announced	2023-11-14: Updated project metadata.

10.1128/MBIO.01633-22;

submitter keyword: Helicobater pylori, antibiotic resistance, bismuth, membrane vesicle

Hilde DE REUSE
contact affiliation	Unité "Pathogenèse de Helicobacter" Department of Microbiology CNRS UMR2001 Institut Pasteur 28 rue du Dr. Roux 75724 Paris cedex 15
contact email	hilde.de-reuse@pasteur.fr
lab head
Magalie Duchateau
contact affiliation	Institut Pasteur
contact email	magalie.duchateau@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD028509

PRIDE project URI

• PRIDE
  1. PXD028509
     1. Label: PRIDE project
     2. Name: H. pylori utilizes membrane vesicles as a novel tool to extrude bismuth drug