PXD028484-1

PXD028484 is an original dataset announced via ProteomeXchange.

Title	Drosophila nicotinic acetylcholine receptor subunits and their native interactions with insecticidal peptide toxins
Description	Description Drosophila melanogaster nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that present a target for insecticides. However, a better understanding of in vioreceptor subunit composition is needed for effective design of insecticides. Alpha-neurotxins are known as peptide toxins capable to block nAChRs by binding to its target subunits. To facilitate the analysis of nAChRs we used a CRISPR/Cas9 strategy to generate null alleles for all ten nAChR subunit genes. We studied interactions of nAChRs to a snake toxin by using styrene maleic acid lipid particles (SMALPs) extraction method. For the null alleles which are impaired in locomotor activities we examined the binding of nAChRs incorporated into SMALPs to alpha bungarotoxin by employing mass spectrometry. We show native ligand interactions to Dalpha5, Dalpha6 and Dalpha7 subunits and reveal glycosylation sites of Dalpha5 and Dalpha7 by a glycoproteomic approach. Finally, we report the localization of fluorescent tagged Dalpha6 across nervous system development
HostingRepository	PRIDE
AnnounceDate	2021-10-12
AnnouncementXML	Submission_2021-10-11_16:03:59.121.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028484
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kathryn Lilley
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-15 14:26:46	ID requested
⏵ 1	2021-10-11 16:03:59	announced

Dataset with its publication pending

submitter keyword: nAChRs, SMALPs, pull-down,toxins

Kathryn S. Lilley
contact affiliation	Cambridge Centre for Proteomics Department of Biochemistry University of Cambridge
contact email	ksl23@cam.ac.uk
lab head
Kathryn Lilley
contact affiliation	University of Cambridge
contact email	ksl23@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028484
     1. Label: PRIDE project
     2. Name: Drosophila nicotinic acetylcholine receptor subunits and their native interactions with insecticidal peptide toxins