PXD028409-1

PXD028409 is an original dataset announced via ProteomeXchange.

Title	The novel peptide ScrA acts through the SaeRS two component system to regulate virulence gene expression in Staphylococcus aureus
Description	Staphylococcus aureus is a Gram-positive opportunistic pathogen, which is carried by approximately 30 % of the population at any time. In addition to being a commensal S. aureus can cause an array of infections, ranging from mild skin and soft tissue infections to life threatening endocarditis and septicemia. S. aureus encodes a variety of virulence factors that facilitate its pathogenic lifestyle. Genes encoding these virulence factors are under tight control by a complex regulatory network, which includes, sigma factors, sRNAs, and protein-based systems, such as Two-Component Systems (TCS). Previous work in our lab demonstrated that overexpression of the sRNA tsr37 leads to an increase in cellular aggregation in the absence of host serum. We determined that the clumping phenotype was dependent on a previously unannotated 88 amino acid protein encoded within the tsr37 sRNA transcript (which we named ScrA for S. aureus clumping regulator A). In addition to increased clumping, overexpression of ScrA also leads to increased biofilm formation. To investigate the mechanism of action of ScrA we performed mass spectrometry proteomics and RNA-sequencing in the ScrA overexpressing strain. A variety of virulence factors, including several surface adhesins were upregulated while several proteases were downregulated. Moreover, results showed a significant upregulation of the SaeRS two component system, suggesting that ScrA is influencing SaeRS activity. We go on to demonstrate that overexpression of ScrA in a saeR mutant abrogates the clumping/biofilm phenotypes confirming that ScrA functions via the Sae system. Finally, we identified the ArlRS TCS as a potential positive regulator of scrA expression. Collectively our results show that ScrA is an activator of the SaeRS system and suggests that ScrA may act as an intermediary between the ArlRS and SaeRS systems.
HostingRepository	PRIDE
AnnounceDate	2022-08-11
AnnouncementXML	Submission_2022-08-11_13:10:45.646.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ronan Carroll
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 09:01:15	ID requested
⏵ 1	2022-08-11 13:10:46	announced

Wittekind MA, Frey A, Bonsall AE, Briaud P, Keogh RA, Wiemels RE, Shaw LN, Carroll RK, The novel protein ScrA acts through the SaeRS two-component system to regulate virulence gene expression in Staphylococcus aureus. Mol Microbiol, 117(5):1196-1212(2022) [pubmed]

submitter keyword: Staphylococcus aureus, Virulence, Small peptides, SaeRS

Ronan Carroll
contact affiliation	Department of Biological Sciences, Ohio University
contact email	carrolr3@ohio.edu
lab head
Ronan Carroll
contact affiliation	Ohio University
contact email	carrolr3@ohio.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028409
     1. Label: PRIDE project
     2. Name: The novel peptide ScrA acts through the SaeRS two component system to regulate virulence gene expression in Staphylococcus aureus