PXD028396-1

PXD028396 is an original dataset announced via ProteomeXchange.

Title	Klebsiella pneumoniae x546,ATCC15380, TMT
Description	Klebsiella pneumoniae x546,ATCC15380, TMT,The samples were ground into a powder in liquid nitrogen. Then the powder was suspended in lysis buffer [1% sodium deoxycholate (SDS), 8M urea]. The mixture was allowed to settle at 4 鎺矯 for 30 min during which the sample were vortexed at every 5 min, and treated by ultrasound at 40 kHz and 40 W for 2 min. After centrifugation at 16000 rpm at 4鎺矯 for 30 min, the concentration of protein supernatant was determined by Bicinchoninic acid (BCA) method by BCA Protein Assay Kit (Pierce, Thermo, USA). Protein quantification was performed according to the kit protocol(Chen et al., 2021)
HostingRepository	PRIDE
AnnounceDate	2021-11-15
AnnouncementXML	Submission_2021-11-15_00:59:38.576.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xin wang
SpeciesList	scientific name: Klebsiella pneumoniae; NCBI TaxID: 573; scientific name: Klebsiella pneumoniae subsp. pneumoniae DSM 30104 = JCM 1662 = NBRC 14940; NCBI TaxID: 1162296; scientific name: Klebsiella pneumoniae MGH 78578; NCBI TaxID: 1328388;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:35:30	ID requested
⏵ 1	2021-11-15 00:59:38	announced

Dataset with its publication pending

submitter keyword: Klebsiella pneumoniae x546,ATCC15380, TMT

Wang Xin
contact affiliation	Tianjin university
contact email	546568836@qq.com
lab head
xin wang
contact affiliation	Tian Jin university
contact email	546568836@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD028396

PRIDE project URI

• PRIDE
  1. PXD028396
     1. Label: PRIDE project
     2. Name: Klebsiella pneumoniae x546,ATCC15380, TMT