PXD028329-2

PXD028329 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of the role of miRNA156 in alfalfa under drought stress
Description	Alfalfa is the most produced perennial forage crop in Canada. Drought stress is a major form of abiotic stress, affecting its productivity and annual yield. A small RNA, miR156, plays a major role in drought tolerance by downregulating downstream SPL genes, but its effects at the proteome level are unknown. In this study, the protein level perturbations of miR156 overexpression (A8) and empty vector (EV) control genotypes were compared under drought stress. Using label-free quantification, 3,000 protein groups were identified, of which 68 were upregulated in A8 and 84 were downregulated relative to EV under control conditions. Conversely, under drought stress, 610 proteins were upregulated and only 52 proteins were downregulated in A8 relative to EV. Functional analysis using PlantRegMap showed that the enriched proteins are likely involved in biological and molecular processes including antioxidant response, response to stress, signal transduction and biosynthesis of secondary metabolites. These proteins/pathways might be involved in the enhancement of drought stress tolerance mediated by miR156. Protein groups related to signaling, such as MAP kinase, calcium-dependent protein kinase, protein phosphatase 2C, and transcriptional regulators including bZIP and zinc finger proteins were found to be differentially expressed when a search was conducted against a drought stress gene database. The proteomic dataset was validated by immunoblotting of selected proteins. The results of this study provide a better understanding and insight into the role of miR156 in drought stress tolerance in alfalfa at the proteomic level.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:16:28.802.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frédéric Marsolais
SpeciesList	scientific name: Medicago sativa (Alfalfa); NCBI TaxID: 3879;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:26:10	ID requested
1	2023-10-19 11:01:09	announced
⏵ 2	2023-11-14 07:16:29	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:06:33	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: drought stress, squamosa promoter-binding protein,2. Alfalfa, proteome., miR156

Frédéric Marsolais
contact affiliation	Agriculture and Agri-Food Canada
contact email	Frederic.Marsolais@agr.gc.ca
lab head
Frédéric Marsolais
contact affiliation	Agriculture and Agri-Food Canada
contact email	frederic.marsolais@agr.gc.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028329
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of the role of miRNA156 in alfalfa under drought stress