PXD028301-1

PXD028301 is an original dataset announced via ProteomeXchange.

Title	Immunoreactive proteins of Echinococcus granulosus revealed by plasma from patients with cystic echinococcosis
Description	The management of cystic echinococcosis (CE) is still problematic, since the post-surgical period is marked by high rates of relapses that, often, could not be early detectable with the available imaging methods. The aim of our study was the search of reliable serological biomarkers for the early prediction of CE post-operative outcomes. Here, we applied an innovative proteomic approach based on immunoprecipitation (IP) of Echinococcus granulosus (E. granulosus) protoscolex antigens with plasma collected at 1-month and 1-year post-surgery, from pediatric CE-confirmed patients, followed by LC-MS/MS. We compared IP proteomic content from patients relapsed within the first-year post-surgery (RCE) to cases with no detectable relapses until 3 post-operative years (NRCE) and selected the most promising candidates. These proteins were produced under recombinant form and assessed for their prognostic performance by ELISA. A total of 305 immunoreactive parasitic proteins were identified, 59 of which, were significantly more abundant in RCE than NRCE for both time-points. We selected four proteins derived from E. granulosus protoscolex as promising prognostic biomarkers for predicting CE post-surgical outcomes: cytoplasmic malate dehydrogenase (Eg-cMDH), citrate synthase (Eg-CS), Annexin A6 (Eg-ANX A6) and severin (Eg-SVN). ELISA results showed that IgG antibodies against the four markers were significantly lower at 1-year post-surgery than 1-month in NRCE, in contrast to RCE group that displayed either a stable or higher IgG levels. The Eg-cMDH and Eg-CS showed the best prognostic performance with respective probabilities of being “relapse-free” of 83% and 81% if a decrease of IgG levels occurred between 1-month and 1-year post-surgery.
HostingRepository	PRIDE
AnnounceDate	2026-09-01
AnnouncementXML	Submission_2026-09-01_09:08:50.892.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thierry Balliau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Echinococcus granulosus; NCBI TaxID: NEWT:6210;
ModificationList	(R)-5-oxo-1; dehydrated residue; acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:19:54	ID requested
⏵ 1	2026-09-01 09:08:51	announced

10.1016/j.jinf.2021.09.023;

Ben Salah E, Barrera C, Sakly W, Mosbahi S, Balliau T, Franche N, Gottstein B, Ben Youssef S, Mekki M, Babba H, Millon L, Novel biomarkers for the early prediction of pediatric cystic echinococcosis post-surgical outcomes. J Infect, 84(1):87-93(2022) [pubmed]

submitter keyword: antigens,Immunoprecipitation, cystic echinococcosis

Laurence Millon
contact affiliation	UMR6249 Chrono-Environnement UFR Sciences médicales et pharmaceutiques 19 rue Ambroise Paré 25030 Besançon
contact email	laurence.millon@univ-fcomte.fr
lab head
Thierry Balliau
contact affiliation	Plateforme d'Analyse Proteomique Paris Sud-Ouest Université Paris-Saclay, INRAE, CNRS, AgroParisTech, GQE - Le Moulon, 91190, Gif-sur-Yvette, France
contact email	thierry.balliau@inrae.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD028301

PRIDE project URI

• PRIDE
  1. PXD028301
     1. Label: PRIDE project
     2. Name: Immunoreactive proteins of Echinococcus granulosus revealed by plasma from patients with cystic echinococcosis