PXD028276-1

PXD028276 is an original dataset announced via ProteomeXchange.

Title	The interferon-inducible antiviral GTPase MxB promotes capsid disassembly and genome release of herpesviruses
Description	Host proteins sense viral products and induce defense mechanisms, particularly in immune cells. Using cell-free assays and quantitative mass spectrometry, we determined the interactomes of capsidhost protein complexes of herpes simplex virus, and identified the large dynamin-like GTPase myxovirus resistance protein B (MxB) as an interferon-inducible protein interacting with capsids. Electron microscopy analyses showed that cytosols containing MxB had the remarkable capability to disassemble the icosahedral capsids of herpes simplex viruses and varicella zoster virus into flat sheets of connected triangular faces. In contrast, capsids remained intact in cytosols with MxB mutants unable to hydrolyze GTP or to dimerize. Our data suggest that MxB senses herpesviral capsids, mediates their disassembly, and thereby restricts the efficiency of nuclear targeting of incoming capsids and/or the assembly of progeny capsids. The resulting premature release of viral genomes from capsids may enhance the activation of DNA sensors, and thereby amplify the innate immune responses.
HostingRepository	PRIDE
AnnounceDate	2022-05-06
AnnouncementXML	Submission_2022-05-06_11:27:03.157.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Virginie Girault
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human alphaherpesvirus 1 strain 17; NCBI TaxID: 10299;
ModificationList	L-methionine sulfoxide; N-acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:09:53	ID requested
⏵ 1	2022-05-06 11:27:03	announced

Dataset with its publication pending

submitter keyword: HSV1

MxB

Host defense

Andreas Pichlmair
contact affiliation	Institute of Virology, Technical University of Munich, Munich 81675, Germany
contact email	andreas.pichlmair@tum.de
lab head
Virginie Girault
contact affiliation	Institute of Virology - Technical University Munich
contact email	v.girault@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028276
     1. Label: PRIDE project
     2. Name: The interferon-inducible antiviral GTPase MxB promotes capsid disassembly and genome release of herpesviruses