PXD028240-1

PXD028240 is an original dataset announced via ProteomeXchange.

Title	Regulatory Mechanisms between Pattern- and Effector-Triggered Immunity in Tomato Leaves Revealed by Integrative Proteomic and Phosphoproteomic Analyses
Description	Plants have evolved a two-layered immune system that mainly includes pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) against pathogen attack. PTI and ETI signaling are functionally linked, but also distinct due to specific perceived ligands and activation modes. Unraveling how PTI and ETI coordinate the immune responses against pathogens is crucial for understanding the regulatory mechanisms in plant immunity. To better understand the protein profiling and phosphorylation events during PTI and ETI, we employed integrated whole proteome and phosphoproteome analyses in the tomato-Pst pathosystem with different Pst DC3000 mutants that allow dissection of different layers of immune responses. A total of 225 proteins and 79 phosphopeptides were differentially regulated in tomato leaves during immune responses. Our proteomics results indicate that some overlapping immune responses are triggered by both PTI and ETI-inducing treatment, and ETI response is more robust than PTI response for most proteins. The change patterns of protein abundance and phosphorylation revealed some key regulators involved in Ca2+ signaling, mitogen-activated protein kinase cascades, and reversible protein phosphorylation, ROS and redox homeostasis, direct defense, transcription machinery and protein turnover, cell wall remodeling, hormone biosynthesis, as well as immune molecule accumulation, are modulated during PTI and/or ETI, suggesting their common or specific roles in plant immune responses. However, NAC domain protein and lipid particle serine esterase, two PTI-specific genes from previous transcriptomic work, have not been detected as differentially regulated in our proteomic analysis, and they were proved to be not PTI-specific inducible and therefore cannot be used as PTI-reporters through “overlapping circle” pattern assay. These results provide insights into the fine-tuned regulatory mechanisms between PTI and ETI in-Pst pathosystem, which will springboard further investigations into the sophisticated mechanisms in plant immunity.
HostingRepository	PRIDE
AnnounceDate	2021-11-25
AnnouncementXML	Submission_2021-11-25_06:43:51.431.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuemei Zhang
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 07:56:39	ID requested
⏵ 1	2021-11-25 06:43:51	announced

Dataset with its publication pending

submitter keyword: tomato, Pst, plant immunity, PTI, ETI, Proteomics, Phosphorylation

Sixue Chen
contact affiliation	Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, USA
contact email	schen@ufl.edu
lab head
Xuemei Zhang
contact affiliation	University of Florida
contact email	zhangxuemei199111@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028240
     1. Label: PRIDE project
     2. Name: Regulatory Mechanisms between Pattern- and Effector-Triggered Immunity in Tomato Leaves Revealed by Integrative Proteomic and Phosphoproteomic Analyses