PXD028193-2

PXD028193 is an original dataset announced via ProteomeXchange.

Title	Inner membrane complex proteomics reveals a palmitoylation cascade regulating intraerythrocytic development of malaria parasite
Description	Malaria is caused by infection of the erythrocytes by the parasites Plasmodium. Inside the erythrocytes, the parasites grow and multiply via schizogony, an unconventional cell division mode. The Inner Membrane Complex (IMC), an organelle located beneath the plasma membrane of the parasites, is essential for schizogony, serving as the platform for protein anchorage. However, the protein compositions of IMC and their localization determinants remain unclear. Here we used biotin ligase (TurboID)-based proximity labelling and quantitative mass spectrometry to compile the proteome of the schizont IMC of rodent malaria parasite Plasmodium yoelii. In total, the IMC proteome consisting of 488 proteins were identified. 19 of the 22 selected candidates were confirmed to localize in the IMC, indicating good reliability. In light of the existing palmitome of Plasmodium falciparum, 136 proteins of the P. yoelii IMC proteome are potentially palmitoylated. We further identified DHHC2 the major resident palmitoyl-acyl-transferase of the IMC. Conditional depletion of DHHC2 led to defective schizont segmentation and growth arrest in vitro and in vivo. DHHC2 was found to palmitoylate two critical IMC proteins CDPK1 and GAP45 for their IMC localization. In summary, this study reports a comprehensive inventory of P. yoelii IMC proteins and demonstrates a central role of DHHC2 governing IMC localization of proteins during the schizont development.
HostingRepository	iProX
AnnounceDate	2022-07-01
AnnouncementXML	Submission_2022-07-20_21:53:54.438.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuanqi Zhong
SpeciesList	scientific name: Plasmodium yoelii yoelii 17XNL; NCBI TaxID: 352914;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-01 07:01:16	ID requested
1	2022-06-30 19:15:29	announced
⏵ 2	2022-07-20 21:53:55	announced	2022-07-21: Update publication information.

Qian P, Wang X, Zhong CQ, Wang J, Cai M, Nguitragool W, Li J, Cui H, Yuan J, Inner membrane complex proteomics reveals a palmitoylation regulation critical for intraerythrocytic development of malaria parasite. Elife, 11():(2022) [pubmed]

submitter keyword: malaria parasite, SWATH-MS, proximity labelling

Jing Yuan
contact affiliation	Xiamen University
contact email	yuanjing@xmu.edu.cn
lab head
Chuanqi Zhong
contact affiliation	Xiamen University
contact email	zhongcq@xmu.edu.cn
dataset submitter

iProX dataset URI