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PXD028162 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegrated Multi-Omics Reveal Polycomb Repressive Complex 2 Restricts Human Trophoblast Induction
DescriptionHuman naive pluripotent stem cells have unrestricted lineage potential. Underpinning this property, naive cells are thought to lack chromatin-based lineage barriers. However, this assumption has not been tested. Here, we apply multi-omics to comprehensively define the chromatin-associated proteome, histone post-translational modifications and transcriptome of human naive and primed pluripotent stem cells. Integrating the chromatin-bound proteome and histone modification data sets reveals differences in the relative abundance and activities of distinct chromatin modules, identifying a strong enrichment of Polycomb Repressive Complex 2 (PRC2)-associated H3K27me3 in naive pluripotent stem cell chromatin. Single-cell approaches and human blastoid models reveal that PRC2 activity acts as a chromatin barrier restricting the differentiation of naive cells towards the trophoblast lineage, and inhibiting PRC2 promotes trophoblast fate induction and cavity formation. Our results establish that human naive pluripotent stem cells are not epigenetically unrestricted, but instead possess chromatin mechanisms that oppose the induction of alternative cell fates. Data originating from the LC-MS/MS analysis of the histone PTMs can be consulted via this project.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMaarten Dhaenens
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonomethylated residue; formylated residue; monohydroxylated residue; acetylated residue; deamidated residue
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-26 08:27:13ID requested
12022-05-06 03:40:17announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Histone post-translational modifications, hPSCs, trophoblast, PRC2, LC-MS/MS
Contact List
Maarten Dhaenens
contact affiliationProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, B-9000 Ghent, Belgium
contact emailmaarten.dhaenens@ugent.be
lab head
Maarten Dhaenens
contact affiliationFaculity of Pharmaceutical Biotechnology
contact emailmaarten.dhaenens@ugent.be
dataset submitter
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Dataset FTP location
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