PXD028161-1

PXD028161 is an original dataset announced via ProteomeXchange.

Title	The E3 ubiquitin ligase FBXL6 controls the quality of newly synthesized mitochondrial ribosomal proteins
Description	Most mitochondrial proteins are synthetized in the cytosol and imported into mitochondria. Here, we demonstrate that the E3 ubiquitin ligase F-box/LRR-repeat protein 6 (FBXL6) controls the quality of newly synthesized mitochondrial ribosomal proteins in mammalian cells. We found that cells lacking FBXL6 display altered mitochondrial metabolism, inhibited cell cycle progression, and present mitochondrial ribosomal protein aggregates. We found that FBXL6 interacts with mitochondrial ribosome precursors, various chaperones, and ribosome-associated quality control proteins and unlike the mitochondrial E3 ubiquitin ligase FBXL4, FBXL6 is located in the cytosol. Our findings demonstrate that FBXL6 is recruited to aggregates containing defective mitochondrial ribosomal proteins and chaperones. Deletion of FBXL6 specifically impaired the degradation of these defective proteins. Hence, our results suggest that FBLX6 participates in the quality control mechanisms of neosynthesized mitochondrial ribosomal proteins in coordination with translation-associated quality control processes.
HostingRepository	PRIDE
AnnounceDate	2025-09-17
AnnouncementXML	Submission_2025-09-17_08:56:03.073.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028161
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Johana Chicher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-26 08:26:50	ID requested
⏵ 1	2025-09-17 08:56:03	announced

10.6019/PXD028161;

10.1016/J.CELREP.2023.112579;

submitter keyword: Human, FBXL6, TurboID, E3 ubiquitin ligase

Patryk Ngondo
contact affiliation	Institut de Biologie Moléculaire des Plantes, UPR 2357 du CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France
contact email	patryk.ngondo@ibmp-cnrs.unistra.fr
lab head
Johana Chicher
contact affiliation	Université de Strasbourg, Plateforme Protéomique Strasbourg-Esplanade, Centre National de la Recherche Scientifique
contact email	j.chicher@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD028161

PRIDE project URI

• PRIDE
  1. PXD028161
     1. Label: PRIDE project
     2. Name: The E3 ubiquitin ligase FBXL6 controls the quality of newly synthesized mitochondrial ribosomal proteins