PXD028160-1

PXD028160 is an original dataset announced via ProteomeXchange.

Title	hnRNPM, ELAVL1, and TBK1 form a type I interferon-inducing multiprotein complex downstream of cGAS and RIG-I
Description	RIG-I like helicases (RLHs) and cGAS are crucial cytosolic sensors of viral RNA and DNA, respectively, and induce type I IFNs via TBK1/ IKKe. hnRNPM was described to possess antimicrobial activities. Here, we show that hnRNPM promotes TBK1/IRF3 phosphorylation and type I IFN induction downstream of cGAS and RIG-I in THP-1 cells and primary human fibroblasts. Interactome analysis revealed that hnRNPM forms a multiprotein complex with ELAVL1, SON, TBK1, IKKe, IKKb, and NFkB p65. The purified hnRNPM complex enhanced TBK1 phosphorylation. Vice versa, TBK1 directly phosphorylated ELAVL1. hnRNPM, ELAVL1, and TBK1 predominantly interacted in the cytosol close to the nuclear membrane. Of note, patients with mutations in SON suffer from recurrent infections, underlining its biologic significance. To our knowledge, hnRNPM, ELAVL1, and SON represent the first non-redundant signaling components merging the cGAS-STING and RIG-I–MAVS pathways. Therefore, our findings may have implications for host defense and auto-inflammatory diseases.
HostingRepository	PRIDE
AnnounceDate	2024-11-11
AnnouncementXML	Submission_2024-11-11_11:53:42.457.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Urban
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-26 08:26:34	ID requested
⏵ 1	2024-11-11 11:53:42	announced
2	2025-01-03 02:16:48	announced	2025-01-03: Updated project metadata.
3	2025-01-06 17:17:58	announced	2025-01-07: Updated project metadata.

Dataset with its publication pending

submitter keyword: AP-MS,LC-MS/MS

Andreas Pichlmair
contact affiliation	Technical University of Munich, School of Medicine, Institute of Virology, 81675 Munich, Germany
contact email	andreas.pichlmair@tum.de
lab head
Christian Urban
contact affiliation	Institute of Virology, Technical University Munich
contact email	c.urban@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD028160

PRIDE project URI

• PRIDE
  1. PXD028160
     1. Label: PRIDE project
     2. Name: hnRNPM, ELAVL1, and TBK1 form a type I interferon-inducing multiprotein complex downstream of cGAS and RIG-I