PXD028128-2

PXD028128 is an original dataset announced via ProteomeXchange.

Title	Proteome characterization of mitochondria, ER, and mitochondrial-associated membrane from BAT and liver
Description	Mitochondria are the provider of cellular energy from aerobic respiration in all tissues. However, only brown adipose tissue (BAT) mitochondria are able to convert chemical energy in the form triglycerides into heat (thermogenesis). To ask if cellular respiration or thermogenic function require a specific lipidomic environment, we investigated the lipid species composition of brown adipose tissue mitochondria, and compared it those purified from liver, skeletal muscle and white adipose tissue. We emplyed mass spectrometry-based proteomics to evalaute the purity of the mitochondia fractions isolated from murine BAT and liver tissue by differential centrifugation, by comparison of its full proteome with endoplasmatic reticulum, mitochondrial-associated membrane, and mitochondria fractions isolated by density gradient centrifugation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:37.699.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Piero Giansanti
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-25 05:42:45	ID requested
1	2024-05-24 02:40:24	announced
⏵ 2	2024-10-22 06:42:38	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: metabolism,proteomics, mass spectrometry, organelles, mitochondria, lipids

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany
contact email	kuster@tum.de
lab head
Piero Giansanti
contact affiliation	Technical University of Munich
contact email	piero.giansanti@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD028128

PRIDE project URI

• PRIDE
  1. PXD028128
     1. Label: PRIDE project
     2. Name: Proteome characterization of mitochondria, ER, and mitochondrial-associated membrane from BAT and liver