PXD028127-2

PXD028127 is an original dataset announced via ProteomeXchange.

Title	Genetic analysis of E3 ubiquitin ligases using CRISPR-Cas9 in primary T cells identifies TRAF2 and UHRF1 as regulators of HIV infection and latency reversal
Description	During the course of HIV infection, host ubiquitination pathways are both attempting to fight off infection and are being hijacked by the virus in an attempt to optimally infect our cells. In order to more fully understand the role ubiquitination plays in HIV infection, we first defined the set of ubiquitin ligases expressed in primary CD4+ T cells using mass spectrometry. Out of the approximately 380 total ligases, we identified 116 that were expressed, and proceeded to delete them individually in primary CD4+ cells using an arrayed CRISPR/Cas9 approach.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:28:38.934.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Danielle Swaney
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-25 04:27:55	ID requested
1	2024-02-06 06:42:01	announced
⏵ 2	2024-10-22 06:28:39	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: HIV, E3 ligase

Nevan Krogan
contact affiliation	UCSF, San Francisco, CA
contact email	daniswan@gmail.com
lab head
Danielle Swaney
contact affiliation	UCSF
contact email	daniswan@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD028127

PRIDE project URI

• PRIDE
  1. PXD028127
     1. Label: PRIDE project
     2. Name: Genetic analysis of E3 ubiquitin ligases using CRISPR-Cas9 in primary T cells identifies TRAF2 and UHRF1 as regulators of HIV infection and latency reversal