PXD028122-1

PXD028122 is an original dataset announced via ProteomeXchange.

Title	Ubiquitination of DEAD-box RNA helicase DDX3X by TRIM25
Description	Tripartite motif protein 25 (TRIM25) is an E3 ligase that ubiquitinates multiple substrates within the RLR signalling cascade and plays both RING (really interesting new gene)-dependent and RING-independent roles in RIG-I-mediated IFN induction. We report that the PRY-SPRY domain of TRIM25 interacts with the N-terminal extension (NTE) of DEAD-box helicase 3X (DDX3X), a host protein with multiple roles in RLR signalling. Gene reporter assays and knockdown studies reveal DDX3X and TRIM25 cooperate to activate the IFN- promoter following RIG-I activation independent of DDX3X’s catalytic activity. We also show that TRIM25 ubiquitinates DDX3X at several lysine residues in vitro and in cells.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_10:19:47.927.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oded Kleifeld
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-25 03:55:45	ID requested
⏵ 1	2021-09-08 10:19:48	announced

Atkinson SC, Heaton SM, Audsley MD, Kleifeld O, Borg NA, TRIM25 and DEAD-Box RNA Helicase DDX3X Cooperate to Regulate RIG-I-Mediated Antiviral Immunity. Int J Mol Sci, 22(16):(2021) [pubmed]

submitter keyword: DDX3X,TRIM25, Ubiquitin

Natalie Borg
contact affiliation	Center Biomol. Struct. & Organization Head, Immunity and Immune Evasion Lab Bachelor of Health and Biomedical Sciences Honours (BH058) Program Manager School of Health and Biomedical Sciences | RMIT University PO Box 71 | Bundoora 3083 | Australia
contact email	natalie.borg@rmit.edu.au
lab head
Oded Kleifeld
contact affiliation	Technion
contact email	okleifeld@technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD028122

PRIDE project URI

• PRIDE
  1. PXD028122
     1. Label: PRIDE project
     2. Name: Ubiquitination of DEAD-box RNA helicase DDX3X by TRIM25