PXD028120 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | O-glycoproteome of Porphyromonas gingivalis |
Description | Porphyromonas gingivalis is an important human pathogen and also a model organism for the Bacteroidetes phylum. O-glycosylation has been reported in this phylum with findings that include the O-glycosylation motif, the structure of the O-glycans in a few species and an extensive O-glycoproteome analysis in Tannerella forsythia. However, O-glycosylation has not yet been confirmed in P. gingivalis. We therefore used glycoproteomics approaches including partial deglycosylation with trifluoromethanesulfonic acid as well as both HILIC and FAIMS based glycopeptide enrichment strategies leading to the identification of 257 putative glycosylation sites in 145 glycoproteins. The sequence of the major O-glycan was elucidated to be Hex-(dHex)-HexA-Hex-dHex-HexNAc-(P-Gro-[Ac]0-2)-HexNAc. Western blot analyses of mutants lacking the glycosyltransferases PGN_1134 and PGN_1135 demonstrated their involvement in the biosynthesis of the glycan while mass spectrometry analysis of the truncated O-glycans suggested that PGN_1134 and PGN_1135 transfer the two HexNAc sugars. Interestingly, a strong bias against the O-glycosylation of abundant proteins exposed to the cell surface such as abundant T9SS cargo proteins, surface lipoproteins and outer membrane β-barrel proteins was observed. In contrast, the great majority of proteins associated with the inner membrane or periplasm were glycosylated irrespective of their abundance. The P. gingivalis O-glycosylation system may therefore function to establish the desired physicochemical properties of the periplasm. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-15 |
AnnouncementXML | Submission_2022-02-15_09:10:04.926.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD028120 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Paul Veith |
SpeciesList | scientific name: Porphyromonas gingivalis ATCC 33277; NCBI TaxID: 431947; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-25 03:55:33 | ID requested | |
⏵ 1 | 2022-02-15 09:10:05 | announced | |
Publication List
Veith PD, Shoji M, Scott NE, Reynolds EC, Characterization of the O-Glycoproteome of Porphyromonas gingivalis. Microbiol Spectr, 10(1):e0150221(2022) [pubmed] |
Keyword List
submitter keyword: Porphyromonas gingivalis, O-glycosylation, Trifluoromethanesulfonic acid, glycan biosynthesis |
Contact List
Eric C Reynolds |
contact affiliation | Melbourne Dental School, Bio21 Institute, University of Melbourne |
contact email | e.reynolds@unimelb.edu.au |
lab head | |
Paul Veith |
contact affiliation | Melbourne Dental School |
contact email | pdv@unimelb.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD028120 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028120
- Label: PRIDE project
- Name: O-glycoproteome of Porphyromonas gingivalis