PXD028087-1

PXD028087 is an original dataset announced via ProteomeXchange.

Title	Dnj4HA AP-MS in Cryptococcus neoformans
Description	Proteins which act as histone chaperones ensure genomic integrity during routine processes such as DNA replication and transcription as well as DNA repair upon damage. Herein, we identify a nuclear J domain protein, Dnj4, which interacts with histones 3 and 4 as well as Hsp71 as demonstrated in an AP-MS experiment suggesting that it integrates the heat shock chaperone machinery into histone chaperoning. In support of this, a deletion mutant lacking DNJ4 had higher levels of DNA damage than the wild type or complemented strains and was hypersensitive to DNA damaging agents. The transcriptional response to DNA damage in a mutant lacking DNJ4 was impaired. Genes related to DNA damage and iron homeostasis functions were up-regulated in the wild type strain in response to hydroxyurea treatment, however their up-regulation was either absent or reduced in a dnj4∆ mutant. Accordingly, excess iron rescued the mutant’s growth in response to hydroxyurea treatment. Iron homeostasis is crucial for virulence in Cryptococcus neoformans, however Dnj4 was found to be dispensable for virulence in a mouse model of cryptococcosis. Finally, we confirmed a conserved role of Dnj4 in histone chaperoning by expressing it in S. cerevisiae and showing that it disrupted endogenous histone chaperoning. Altogether, this study highlights the importance of a JDP co-chaperone in histone chaperoning in C. neoformans.
HostingRepository	PRIDE
AnnounceDate	2021-12-15
AnnouncementXML	Submission_2021-12-15_02:47:12.204.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Linda Horianopoulos
SpeciesList	scientific name: Cryptococcus neoformans var. grubii H99; NCBI TaxID: 235443;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-24 03:08:27	ID requested
⏵ 1	2021-12-15 02:47:12	announced

Dataset with its publication pending

submitter keyword: Cryptococcus neoformans, J domain protein, co-chaperone, histone

James W. Kronstad
contact affiliation	Michael Smith Laboratories, University of British Columbia, Canada
contact email	kronstad@msl.ubc.ca
lab head
Linda Horianopoulos
contact affiliation	University of British Columbia
contact email	horianol@msl.ubc.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028087
     1. Label: PRIDE project
     2. Name: Dnj4HA AP-MS in Cryptococcus neoformans