PXD028082-1

PXD028082 is an original dataset announced via ProteomeXchange.

Title	Bioinformatics and mass spectrometry-based proteomics reveal alcohol dehydrogenase 1B as a blood biomarker candidate to monitor acetaminophen-induced liver injury
Description	Acute liver injury (ALI) is a severe disorder resulting from excessive hepatocyte cell death and frequently caused by acetaminophen intoxication. Clinical management of ALI progression is hampered by the dearth of blood biomarkers available. In this study, a bioinformatics workflow was developed to screen Omics databases and identify potential biomarkers for hepatocyte cell death. Then, discovery proteomics was harnessed to select among these candidates those that were specifically detected into the blood of acetaminophen-induced ALI patients. Among these candidates, the isoenzyme alcohol dehydrogenase 1B (ADH1B) was massively leaked into the blood. To evaluate ADH1B, we developed a targeted proteomics assay and quantified ADH1B in serum samples collected at different times from 17 patients admitted for acetaminophen-induced ALI. Serum ADH1B concentrations increased markedly during the acute phase of the disease and dropped to undetectable levels during recovery. In contrast to alanine aminotransferase activity, the rapid drop in circulating ADH1B concentrations was followed by an improvement of the in-ternational normalized ratio (INR) within 10 to 48h and was associated with a favorable outcome. In conclusion, the combination of Omics data exploration and proteomics revealed ADH1B as a new blood biomarker which could be useful to improve the monitoring of acetaminophen-induced ALI.
HostingRepository	PRIDE
AnnounceDate	2021-11-30
AnnouncementXML	Submission_2021-11-30_05:12:57.757.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028082
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hesse Anne-Marie
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-24 02:31:18	ID requested
⏵ 1	2021-11-30 05:12:58	announced

Dataset with its publication pending

submitter keyword: proteomics

mass spectrometry

liver

blood

biomarker

acetaminophen

Virginie Brun
contact affiliation	Univ. Grenoble Alpes, CEA, Inserm, IRIG, BioSanté U1292, Grenoble, France
contact email	virginie.brun@cea.fr
lab head
Hesse Anne-Marie
contact affiliation	Laboratoire d'Etude de la Dynamique des Protéomes
contact email	anne-marie.hesse@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028082
     1. Label: PRIDE project
     2. Name: Bioinformatics and mass spectrometry-based proteomics reveal alcohol dehydrogenase 1B as a blood biomarker candidate to monitor acetaminophen-induced liver injury