PXD028072 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification and function of components from a divergent mRNA export pathway in trypanosomes |
Description | mRNA export is central to gene regulation and expression. In trypanosomes, transcription is polycistronic, all mRNAs are processed by trans-splicing and export is mediated by non-canonical mechanisms. We have described several conserved mRNA export pathway components in Trypanosoma cruzi, including orthologs of Sub2, a component of the TREX complex, and eIF4AIII, a core component of the exon junction complex (EJC). Few orthologs of proteins involved in mRNA export in higher eukaryotes are detectable in trypanosome genomes and examples of mechanistic divergence well known. To uncover additional components of the trypanosome mRNA maturation and export system we undertook an unbiased search for protein interactors of TcSub2 and TceIF4AIII. Significant overlap between TcSub2 and TceIF4AIII interacting protein cohorts suggests that both proteins associate with similar machinery. We confirmed several interactions with conserved core components of the EJC and multiple additional complexes, together with identification of proteins specific to trypanosomatids. The highly interactive super-interactome uncovered here, capable of supporting RNA processing from splicing through to nuclear export and cytoplasmic events, highlights kinetoplastid-specific and conserved components creating an amalgam to support the unique mRNA maturation mechanisms in trypanosomes. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-07 |
AnnouncementXML | Submission_2022-02-07_08:21:54.491.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Patricia Domingues |
SpeciesList | scientific name: Trypanosoma cruzi Dm28c; NCBI TaxID: 1416333; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap XL ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-23 07:49:26 | ID requested | |
1 | 2022-02-07 08:16:27 | announced | |
⏵ 2 | 2022-02-07 08:21:55 | announced | 2022-02-07: Updated publication reference for PubMed record(s): 35091090. |
Publication List
Inoue AH, Domingues PF, Serpeloni M, Hiraiwa PM, Vidal NM, Butterfield ER, Del Pino RC, Ludwig A, Boehm C, Field MC, Á, vila AR, Proteomics Uncovers Novel Components of an Interactive Protein Network Supporting RNA Export in Trypanosomes. Mol Cell Proteomics, 21(3):100208(2022) [pubmed] |
Keyword List
submitter keyword: mRNA export, mRNA processing, EJC, TREX complex, trypanosomes, gene expression, proteomics, evolution, LC-MS/MS |
Contact List
Andréa Rodrigues Ávila |
contact affiliation | Laboratório de Pesquisa em Apicomplexa - LAPAPI, Instituto Carlos Chagas - Fiocruz Paraná |
contact email | andrea.avila@fiocruz.br |
lab head | |
Patricia Domingues |
contact affiliation | Carlos Chagas Institute |
contact email | pfdominguess@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028072
- Label: PRIDE project
- Name: Identification and function of components from a divergent mRNA export pathway in trypanosomes