PXD028040-1

PXD028040 is an original dataset announced via ProteomeXchange.

Title	Patch-Clamp Proteomics of Single Neurons in Tissue Using Electrophysiology and Subcellular Capillary Electrophoresis Mass Spectrometry
Description	In this project, we enabled mass spectrometry based proteomics characterization of single neurons in sections of the mouse brain. We accessed cells in a whole-cell patch clamp configuration and aspirated a portion of the neuronal soma into the patch clamp probe. The collected aspirate was expelled into a microvial, where proteins were extracted and processed for shot-gun analysis. The resulting trace amounts of protein digests were analyzed on a custom-built microanalytical capillary electrophoresis platform that was connected to an electrospray ionization high-resolution mass spectrometer. This “patch proteomics” technology identified ~150 proteins from triplicate measurement of single dopaminergic neurons in the mouse substantia nigra.
HostingRepository	PRIDE
AnnounceDate	2022-02-18
AnnouncementXML	Submission_2022-02-18_15:30:12.856.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Nemes
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-19 21:59:45	ID requested
⏵ 1	2022-02-18 15:30:13	announced
2	2023-11-14 08:53:27	announced	2023-11-14: Updated project metadata.

10.1021/ACS.ANALCHEM.1C03826;

submitter keyword: electrophysiology, mass spectrometry, nervous system, bottom-up proteomics, capillary electrophoresis,Whole-cell patch-clamp, mouse

Peter Nemes
contact affiliation	Department of Chemistry & Biochemistry, University of Maryland, College Park, MD, USA
contact email	Nemes@umd.edu
lab head
Peter Nemes
contact affiliation	University of Maryland, College Park
contact email	nemes@umd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD028040

PRIDE project URI

• PRIDE
  1. PXD028040
     1. Label: PRIDE project
     2. Name: Patch-Clamp Proteomics of Single Neurons in Tissue Using Electrophysiology and Subcellular Capillary Electrophoresis Mass Spectrometry