PXD028014 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Differential protein expression profiles of testes in Tmprss12-/- and wild-type mice |
Description | Serine protease is considered a relative upstream regulator in the physiological processes including male reproduction. Transmembrane serine protease 12 (TMPRSS12) has been shown to regulate sperm motility and uterotubal junction migration in mice, but its role in the testis remains unknown.In this study, we confirmed the important role of TMPRSS12 in spermatogenesis. To further investigate the mechanism of TMPRSS12 in male reproduction, we used 2-D electrophoresis combined with Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry(MALDI-TOF/TOF) to construct differential protein expression profiles of testes in Tmprss12-/- and WT mice. A total of 64 spots with 2-fold or more differential expression between two groups were identified using MALDI-TOF/TOF. As a result, 54 unique proteins were identified successfully, including 39 proteins with decreased expression and 15 proteins with increased expression in Tmprss12-/- mice. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-20 |
AnnouncementXML | Submission_2022-05-20_03:42:00.228.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xinli Zhou |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | ultraflex |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-18 08:50:17 | ID requested | |
⏵ 1 | 2022-05-20 03:42:01 | announced | |
Publication List
Zhang J, Zhou X, Wan D, Yu L, Chen X, Yan T, Wu Z, Zheng M, Zhu F, Zhu H, TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice. Front Cell Dev Biol, 10():757042(2022) [pubmed] |
Keyword List
submitter keyword: TMPRSS12, serine protease, testis |
Contact List
Hui Zhu |
contact affiliation | State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China |
contact email | njzhuhui@njmu.edu.cn |
lab head | |
Xinli Zhou |
contact affiliation | Nanjing Medical University |
contact email | xzxznsyz@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028014
- Label: PRIDE project
- Name: Differential protein expression profiles of testes in Tmprss12-/- and wild-type mice