PXD028002-1

PXD028002 is an original dataset announced via ProteomeXchange.

Title	Mapping protein interactions in the active TOM-TIM23 supercomplex
Description	Nuclear-encoded mitochondrial proteins destined for the matrix have to be transported across two membranes. The TOM and TIM23 complexes facilitate the transport of precursor proteins with N-terminal targeting signals into the matrix. During transport, precursors are recognized by the TIM23 complex in the inner membrane for handover from the TOM complex. However, we have little knowledge on the organisation of the TOM-TIM23 transition zone and on how precursor transfer between the translocases occurs. Here, we designed a precursor protein that is stalled during matrix transport in a TOM-TIM23-spanning manner and enables purification of the translocation intermediate. Combining chemical cross-linking with mass spectrometric analyses and structural modelling allowed us to map the molecular environment of the intermembrane space interface of TOM and TIM23 as well as the import motor interactions with amino acid resolution. Our analyses provide a framework for understanding presequence handover and translocation during matrix protein transport.
HostingRepository	PRIDE
AnnounceDate	2021-12-13
AnnouncementXML	Submission_2021-12-12_16:18:28.620.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olexandr Dybkov
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive HF; Q Exactive HF-X; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-18 02:31:10	ID requested
⏵ 1	2021-12-12 16:18:28	announced
2	2023-11-14 08:53:09	announced	2023-11-14: Updated project metadata.

10.1038/S41467-021-26016-1;

submitter keyword: Saccharomyces cerevisiae, mitochondria, translocation, TIM, TOM23, crosslinking, structural modelling, LC-MSMS

Prof. Dr. Henning Urlaub
contact affiliation	Max-Planck-Institute for Biophysical Chemistry Bioanalytical Mass Spectrometry Group Am Fassberg 11, Goettingen, Germany ************************************************ University Medical Center Goettingen Bioanalytics Institute for Clinical Chemistry Robert Koch Strasse 40, Goettingen, Germany
contact email	henning.urlaub@mpibpc.mpg.de
lab head
Olexandr Dybkov
contact affiliation	Max Planck Institute for biophysical chemistry
contact email	odybkov@gwdg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD028002

PRIDE project URI

• PRIDE
  1. PXD028002
     1. Label: PRIDE project
     2. Name: Mapping protein interactions in the active TOM-TIM23 supercomplex