PXD028001-1

PXD028001 is an original dataset announced via ProteomeXchange.

Title	A deep-sea bacterium senses and utilizes blue light via a BLUF-dependent pathway
Description	Light was a ubiquitous environmental stimulus. Deep-sea microorganisms were exposed to a pervasive blue light optical environment. The utilization of blue light by deep-sea microorganisms, especially non-photosynthetic microorganisms, and the downstream pathway after light reception were obscure. Under the enrichment condition surrounded by blue light, a potential novel species named Spongiibacter nanhainus CSC3.9 from the deep-sea cold seep was isolated. Its growth and metabolism under blue light were significantly better than other wavelengths of light. Six blue light sensing proteins, including four BLUF (Blue Light Using Flavin) and two bacteriophytochrome, were annotated in the genome of strain CSC3.9. Then, with the assist of proteomic analysis, we demonstrated that 15960-BLUF was a crucial blue light receptor that interfered with motor behavior through chemotaxis pathway by means of in vivo and in vitro verification. In addition, 15960-BLUF mediated part of the blue light to promote the growth of strain CSC3.9. Further, we summarized the functional BLUF proteins from isolated marine microorganisms, and the high abundance distribution of BLUF similar to the downstream unresponsive domain type in strain CSC3.9 was demonstrated. The widespread distribution of BLUF protein in marine bacteria implied the extensiveness of this regulatory mechanism, and wavelength variation of light was a potential means to isolate uncultured microorganisms. This was the first reported in deep-sea microorganisms that BLUF-dependent physiological response to blue light. It provided a new clue for the blue light adaptation of microorganisms in disphotic zone.
HostingRepository	PRIDE
AnnounceDate	2022-01-18
AnnouncementXML	Submission_2022-01-18_03:59:49.597.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yeqi shan
SpeciesList	scientific name: Spongiibacter sp. CSC3.9; NCBI TaxID: 2794344;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-18 02:31:01	ID requested
⏵ 1	2022-01-18 03:59:49	announced

Dataset with its publication pending

submitter keyword: deep-sea bacterium, blue light, BLUF, light sensing, chemotaxis

yeqi shan
contact affiliation	The Institute of Oceanology, Chinese Academy of Sciences
contact email	shanyeqi@qdio.ac.cn
lab head
yeqi shan
contact affiliation	The Institute of Oceanology, Chinese Academy of Sciences
contact email	shanyeqi@qdio.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028001
     1. Label: PRIDE project
     2. Name: A deep-sea bacterium senses and utilizes blue light via a BLUF-dependent pathway