PXD027971-1

PXD027971 is an original dataset announced via ProteomeXchange.

Title	Simple, single-shot phosphoproteomic analysis of heat-stable tau
Description	We developed a simple, antibody-free approach for single shot analysis of tau phosphorylation across the entire protein by liquid-chromatography tandem mass spectrometry (LC-MS/MS) to study age-related changes in tau phosphorylation. This methodology is species independent; thus, while initially developed in a rodent model, we utilized this technique to analyze 36 phosphorylation sites on rhesus monkey tau from the prefrontal cortex (PFC), a region vulnerable to AD degeneration. We identified novel, age-related changes in tau phosphorylation in the rhesus monkey PFC and analyzed patterns of phosphorylation change across domains of the protein. We confirmed a significant increase and positive correlation with age of phosphorylated serine235 tau and phosphorylated serine396 tau levels in an expanded cohort of 14 monkeys.
HostingRepository	PRIDE
AnnounceDate	2021-11-25
AnnouncementXML	Submission_2021-11-25_08:51:25.430.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shannon Leslie
SpeciesList	scientific name: Macaca mulatta; NCBI TaxID: 9544; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-17 04:05:46	ID requested
⏵ 1	2021-11-25 08:51:25	announced

Dataset with its publication pending

submitter keyword: phosphoproteomics, tau, brain, heat-stable

Angus Nairn
contact affiliation	Department of Psychiatry, Yale University School of Medicine, New Haven, CT
contact email	angus.nairn@yale.edu
lab head
Shannon Leslie
contact affiliation	Yale University
contact email	shannon.leslie@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD027971

PRIDE project URI

• PRIDE
  1. PXD027971
     1. Label: PRIDE project
     2. Name: Simple, single-shot phosphoproteomic analysis of heat-stable tau