PXD027957-1

PXD027957 is an original dataset announced via ProteomeXchange.

Title	Whole blood peptide assay stability by MRM-MS
Description	Here, we investigate how delays in plasma processing affect peptide-centric “bottom-up” proteomics, using multiple reaction monitoring mass spectrometry (MRM-MS). We used validated Tier 2-level assays for proteotypic peptide surrogates of 270 human proteins to analyze plasma generated from whole blood that has been kept at room temperature from 0-40 hours before plasma generation. Moreover, we evaluated whether different plasma thawing conditions had an impact on the MRM-based plasma protein measurements.
HostingRepository	PanoramaPublic
AnnounceDate	2022-08-18
AnnouncementXML	Submission_2022-08-18_10:58:27.515.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Claudia Gaither
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	6495B Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-16 18:12:11	ID requested
⏵ 1	2022-08-18 10:58:28	announced

Gaither C, Popp R, Zahedi RP, Borchers CH, Multiple Reaction Monitoring-Mass Spectrometry Enables Robust Quantitation of Plasma Proteins Regardless of Whole Blood Processing Delays That May Occur in the Clinic. Mol Cell Proteomics, 21(5):100212(2022) [pubmed]

submitter keyword: whole blood, mass spectrometry, MRM, plasma, proteomics, internal standards

Christoph Borchers
contact affiliation	Segal Cancer Proteomics Centre, McGill University
contact email	christoph.borchers@mcgill.ca
lab head
Claudia Gaither
contact affiliation	MRM Proteomics Inc.
contact email	cgaither@mrmproteomics.com
dataset submitter

Panorama Public dataset URI