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PXD027957 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleWhole blood peptide assay stability by MRM-MS
DescriptionHere, we investigate how delays in plasma processing affect peptide-centric “bottom-up” proteomics, using multiple reaction monitoring mass spectrometry (MRM-MS). We used validated Tier 2-level assays for proteotypic peptide surrogates of 270 human proteins to analyze plasma generated from whole blood that has been kept at room temperature from 0-40 hours before plasma generation. Moreover, we evaluated whether different plasma thawing conditions had an impact on the MRM-based plasma protein measurements.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterClaudia Gaither
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListLabel:13C(6)15N(2); Label:13C(6)15N(4)
Instrument6495B Triple Quadrupole LC/MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-16 18:12:11ID requested
12022-08-18 10:58:28announced
Publication List
Gaither C, Popp R, Zahedi RP, Borchers CH, Multiple Reaction Monitoring-Mass Spectrometry Enables Robust Quantitation of Plasma Proteins Regardless of Whole Blood Processing Delays That May Occur in the Clinic. Mol Cell Proteomics, 21(5):100212(2022) [pubmed]
Keyword List
submitter keyword: whole blood, mass spectrometry, MRM, plasma, proteomics, internal standards
Contact List
Christoph Borchers
contact affiliationSegal Cancer Proteomics Centre, McGill University
contact emailchristoph.borchers@mcgill.ca
lab head
Claudia Gaither
contact affiliationMRM Proteomics Inc.
contact emailcgaither@mrmproteomics.com
dataset submitter
Full Dataset Link List
Panorama Public dataset URI