PXD027954 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Substrate profiling of mitochondrial caseinolytic protease P via a site-specific photocrosslinking approach |
Description | The function of proteases is mainly determined by the substrates they are processing. However, substrate identification remains a challenging task. Here, we used genetic code expansion to incorporate a diazirine-lysine amino acid (DiazK) at selected positions of mitochondrial caseinolytic protease P (ClpP) in mammalian cells and determined its substrate scope by proteomic analyses after UV-light dependent photocrosslink. The results confirm its major involvement in maintaining the overall mitochondrial protein homeostasis as evident from alternative approaches. Additionally, novel putative substrates were identified revealing the highly complementary nature of this approach. Furthermore, this allows for the identification of changing substrate dynamics in an unbiased manner. Induction of oxidative stress with rotenone followed by identification of unique proteins corroborate its role in mitochondrial stress response. We hypothesize, that the oxidative stress is counteracted by processing proteins, which are involved in respiratory chain complex proteins synthesis and maturation as well as catabolic enzymes respectively. |
HostingRepository | PRIDE |
AnnounceDate | 2023-08-22 |
AnnouncementXML | Submission_2023-08-21_21:48:05.820.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | ThomasGronauer |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-16 06:20:01 | ID requested | |
⏵ 1 | 2023-08-21 21:48:21 | announced | |
2 | 2023-11-14 09:04:54 | announced | 2023-11-14: Updated project metadata. |
Publication List
Nguyen TA, Gronauer TF, Nast-Kolb T, Sieber SA, Lang K, Substrate Profiling of Mitochondrial Caseinolytic Protease P via a Site-Specific Photocrosslinking Approach. Angew Chem Int Ed Engl, 61(10):e202111085(2022) [pubmed] |
Keyword List
submitter keyword: mtUPR, Mitochondria, photoaffinity crosslinking,ClpXP, Orbitrap Fusion |
Contact List
Stephan AxelSieber |
contact affiliation | Technical University of Munich, Department of Chemistry, Chair of Organic Chemistry 2, Germany |
contact email | stephan.sieber@tum.de |
lab head | |
ThomasGronauer |
contact affiliation | TU Munich Department of Chemistry Chair of Organic Chemistry 2 Lichtenbergstraße 4 85748 Garching Germany |
contact email | thomas.gronauer@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027954
- Label: PRIDE project
- Name: Substrate profiling of mitochondrial caseinolytic protease P via a site-specific photocrosslinking approach