PXD027944-1

PXD027944 is an original dataset announced via ProteomeXchange.

Title	Analysing alpha4 and alpha9 integrin complex partners
Description	Adhesion of basal keratinocytes to the underlying extracellular matrix (ECM) plays a key role in the control of skin homeostasis and response to injury. Integrin receptors indirectly link the ECM to the cell cytoskeleton through large protein complexes called focal adhesions (FA). FA also function as intracellular biochemical signaling platforms to enable cells to respond to changing extracellular cues. The α4β1 and α9β1 integrins are both expressed in basal keratinocytes, share some common ECM ligands, and have been shown to promote wound healing in vitro and in vivo. However, their roles in maintaining epidermal homeostasis and relative contributions to pathological processes in the skin remain unclear. We found that α4β1 and α9β1 occupied distinct regions in monolayers of a basal keratinocyte cell line (NEB-1). During collective cell migration (CCM), α4 and α9 integrins co-localized along the leading edge. Pharmacological inhibition of α4β1 and α9β1 integrins increased keratinocyte proliferation and induced a dramatic change in cytoskeletal remodeling and FA rearrangement, detrimentally affecting collective cell migration (CCM). Further analysis revealed that α4β1/α9β1 integrins suppress ERK1/2 activity to control migration through the regulation of downstream kinases including Mitogen and Stress Activated Kinase 1 (MSK1). We performed LC-MS/MS analysis of α4β1/α9β1 adhesion complexes to identify partners that could regulate ERK activity.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_06:14:02.890.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027944
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Willow Hight-Warburton
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-16 06:00:12	ID requested
⏵ 1	2022-02-17 06:14:03	announced

Hight-Warburton W, Felix R, Burton A, Maple H, Chegkazi MS, Steiner RA, McGrath JA, Parsons M, 9 Integrins Coordinate Epithelial Cell Migration Through Local Suppression of MAP Kinase Signaling Pathways. Front Cell Dev Biol, 9():750771(2021) [pubmed]

submitter keyword: Human, keratinocytes, LC-MS/MS

Maddy Parsons
contact affiliation	Parsons Group Randall Centre for Cell & Molecular Biophysics King's College London UK
contact email	maddy.parsons@kcl.ac.uk
lab head
Willow Hight-Warburton
contact affiliation	King's College London
contact email	willow.h.hight-warburton@kcl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027944

PRIDE project URI

• PRIDE
  1. PXD027944
     1. Label: PRIDE project
     2. Name: Analysing alpha4 and alpha9 integrin complex partners