PXD027874 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Vibrio natriegens Systems & Synthetic Biology: Proteome Profile (Temperature & Salinity) |
Description | Vibrio natriegens is a rapidly growing salt marsh bacterium that is being developed as a synthetic biology chassis. We characterized its physiological response to different salinities and temperatures in order to optimize culturing conditions and understand its adaptations to a salt marsh environment. Using metabolomics, transcriptomics, and proteomics we determined what pathways respond to these environmental parameters. We found that organic osmolyte synthesis and membrane transporters were most responsive to changes in salinity. The primary osmolytes were glutamate, glutamine, and ectoine, responding to salinity across temperature treatments. However, when media was supplemented with choline, glycine betaine seemed to mostly replace ectoine. These results provide a baseline dataset of metabolic activity under a variety of conditions that will inform decisions made about culturing and genome engineering for future applications. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-15 |
AnnouncementXML | Submission_2021-09-15_13:04:14.588.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Judson Hervey |
SpeciesList | scientific name: Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759; NCBI TaxID: 1219067; |
ModificationList | acetylated residue; monohydroxylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-11 22:13:49 | ID requested | |
⏵ 1 | 2021-09-15 13:04:14 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Synthetic Biology, nanoLC-MS/MS, proteome, proteomics, Vibrio, Vibrio natriegens |
Contact List
J Hervey |
contact affiliation | CBMSE |
contact email | hervey.judson@gmail.com |
lab head | |
Judson Hervey |
contact affiliation | Naval Research Laboratory |
contact email | judson.hervey@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027874
- Label: PRIDE project
- Name: Vibrio natriegens Systems & Synthetic Biology: Proteome Profile (Temperature & Salinity)