PXD027806-1

PXD027806 is an original dataset announced via ProteomeXchange.

Title	The life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts: heavy-female isotopic labeling to estimate abundances of female-derived sperm protein
Description	Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used sex-specific isotopic labeling to estimate the relative abundance of female-derived sperm proteins after long-term storage in the female’s sperm storage organs. In combination with whole-cell quantitative proteomics to track the protein composition of sperm across three female reproductive tissues (bursa, seminal receptacle, and spermatheca) and three post-mating timepoints (30 minutes, 2 hours, and 4 days), as well as an expansion of the seminal vesicle sperm proteome, our data provide a comprehensive, quantitative analysis of the molecular life history of Drosophila sperm.
HostingRepository	PRIDE
AnnounceDate	2022-03-14
AnnouncementXML	Submission_2022-03-14_06:46:23.690.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027806
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Erin McCullough
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-09 06:33:19	ID requested
⏵ 1	2022-03-14 06:46:24	announced

McCullough EL, Whittington E, Singh A, Pitnick S, Wolfner MF, Dorus S, sperm involves molecular continuity between male and female reproductive tracts. Proc Natl Acad Sci U S A, 119(11):e2119899119(2022) [pubmed]

submitter keyword: Drosophila melanogaster, female reproductive tract, fertility, sperm, SILAC, LC-MSMS

Steve Dorus
contact affiliation	Center for Reproductive Evolution, Biology Department, Syracuse University
contact email	sdorus@syr.edu
lab head
Erin McCullough
contact affiliation	Syracuse University
contact email	mccullough.e@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD027806

PRIDE project URI

• PRIDE
  1. PXD027806
     1. Label: PRIDE project
     2. Name: The life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts: heavy-female isotopic labeling to estimate abundances of female-derived sperm protein